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A versatile two-step CRISPR- and RMCE-based strategy for efficient genome engineering in Drosophila

bioRxiv

Aug 11, 2014

Xu Dong ZhangFrank Schnorrer

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Abstract

The development of CRISPR/Cas9 technologies promises a quantum leap in genome-engineering of model organisms. However, CRISPR-mediated gene targeting reports in Drosophila are still restricted to a few genes, use variable experimental conditions and vary in efficiency, questioning the u...read more

Mentioned in this Paper

DNA Sequence
BAT Loci
CRISPR-Cas Systems
Homologous Gene
Mutant
Gene Mutant
Genome
Diptera
Quantum
Gene Targeting
17
31
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  • A versatile two-step CRISPR- and RMCE-based strategy for efficient genome engineering in Drosophila

    bioRxiv

    Aug 11, 2014

    Xu Dong ZhangFrank Schnorrer

    PMID: 990007864

    DOI: 10.1101/007864

    Abstract

    The development of CRISPR/Cas9 technologies promises a quantum leap in genome-engineering of model organisms. However, CRISPR-mediated gene targeting reports in Drosophila are still restricted to a few genes, use variable experimental conditions and vary in efficiency, questioning the u...read more

    Mentioned in this Paper

    DNA Sequence
    BAT Loci
    CRISPR-Cas Systems
    Homologous Gene
    Mutant
    17
    31

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