PMID: 6389920Nov 1, 1984Paper

A very sensitive direct radioimmunoassay system for plasma angiotensin II and its clinical application in various hypertensive diseases

Japanese Circulation Journal
K ShimamotoO Iimura

Abstract

A very sensitive and simplified direct radioimmunoassay system for plasma angiotensin II was developed using the antiserum against synthetic angiotensin II (final dilution = 1: 1,500,000) in combination with 125I-labeled angiotensin II (specific activity = 1,600 microCi/micrograms). In this assay system, it was possible to carry out a direct assay using 100 microliter of plasma without any extraction procedure. This conclusion was supported by 100% recovery, parallelism of plasma samples against the standard curve, and no difference in hormone levels, there was also a high positive correlation between the plasma angiotensin II levels measured by this direct assay and the dowex column extraction method. The sensitivity of this assay system was 0.1 pg/tube, which is the highest sensitivity in studies reported to date. The cross-reactivities of angiotensin III and I against this antiserum were 100% and less than 0.1%, respectively, suggesting that the antiserum was very specific for the C-terminal of angiotensin II. Plasma angiotensin II levels in normal subjects after overnight fasting ranged from 3.0 to 21.3 pg/ml (12.0 +/- 2.1 pg/ml, mean +/- SE). By comparison, plasma angiotensin II levels of patients with essential hypertensi...Continue Reading

Citations

Jun 20, 1996·European Journal of Pharmacology·S MiyataK Kikuchi
May 6, 1998·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·T Zenteno-Savin, M A Castellini
Jan 1, 1988·Clinical and Experimental Hypertension. Part A, Theory and Practice·H MurataniK Fukiyama
Jan 1, 1987·Clinical and Experimental Hypertension. Part A, Theory and Practice·K OmataK Yoshinaga
Jan 1, 1987·Clinical and Experimental Hypertension. Part A, Theory and Practice·S TanakaA Masuda
Jan 1, 1987·Clinical and Experimental Hypertension. Part A, Theory and Practice·M KawamuraT Omae
Jan 1, 1990·Clinical and Experimental Hypertension. Part A, Theory and Practice·H MurataniK Fukiyama
Jul 15, 2005·Kidney International·Osamu TakaseTakao Saruta
Oct 19, 2006·Biochemical and Biophysical Research Communications·Sayaka NagataKazuo Kitamura
Feb 1, 1989·Clinical and Experimental Pharmacology & Physiology·H RakugiY Kumahara
Jan 1, 1993·Clinical and Experimental Pharmacology & Physiology·R MorishitaT Ogihara
Mar 1, 1993·Clinical and Experimental Pharmacology & Physiology·F NakamuraT Ogihara
Nov 1, 1990·Clinical and Experimental Pharmacology & Physiology·H SaitoT Inagami
Nov 1, 1993·Circulation Research·N TomitaT Ogihara
Sep 1, 1995·Clinical and Experimental Pharmacology & Physiology·R MorishitaH Yu
Mar 20, 2020·Scandinavian Journal of Clinical and Laboratory Investigation·Yu FuWei Liu
Jan 1, 1988·Archives of Andrology·A OkuyamaM Matsuda
May 6, 1998·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·T Zenteno-Savin, M A Castellini

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Epigenetics Insights from Twin Studies

Find the latest research on epigenetics and twin studies here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.