A Vibrio cholerae classical TcpA amino acid sequence induces protective antibody that binds an area hypothesized to be important for toxin-coregulated pilus structure

Infection and Immunity
Ronald K TaylorW F Wade

Abstract

Vibrio cholerae is a gram-negative bacterium that has been associated with cholera pandemics since the early 1800s. Whole-cell, killed, and live-attenuated oral cholera vaccines are in use. We and others have focused on the development of a subunit cholera vaccine that features standardized epitopes from various V. cholerae macromolecules that are known to induce protective antibody responses. TcpA protein is assembled into toxin-coregulated pilus (TCP), a type IVb pilus required for V. cholerae colonization, and thus is a strong candidate for a cholera subunit vaccine. Polypeptides (24 to 26 amino acids) in TcpA that can induce protective antibody responses have been reported, but further characterization of their amino acid targets relative to tertiary or quaternary TCP structures has not been done. We report a refinement of the TcpA sequences that can induce protective antibody. One sequence, TcpA 15 (residues 170 to 183), induces antibodies that bind linear TcpA in a Western blot as well as weakly bind soluble TcpA in solution. These antibodies bind assembled pili at high density and provide 80 to 100% protection in the infant mouse protection assay. This is in sharp contrast to other anti-TcpA peptide sera (TcpA 11, TcpA 1...Continue Reading

References

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Citations

Jun 15, 2007·Infection and Immunity·Johanna K HansenKatrina T Forest
Jun 22, 2006·Journal of Bacteriology·Sophie de BentzmannAlain Filloux
Jul 14, 2009·Journal of Bacteriology·Raquel M MartinezRonald K Taylor
Sep 20, 2006·Journal of Molecular Microbiology and Biotechnology·Johanna K Hansen, Katrina T Forest
Aug 10, 2011·Critical Reviews in Microbiology·Chandrika Murugaiah
May 23, 2012·FEMS Immunology and Medical Microbiology·Meghan MuseWilliam F Wade
Feb 16, 2017·Proceedings of the National Academy of Sciences of the United States of America·Eun Jin KimDong Wook Kim

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