A Viral Toolbox of Genetically Encoded Fluorescent Synaptic Tags

IScience
Seth BensussenXue Han

Abstract

Fibronectin intrabodies generated with mRNA display (FingRs) are a recently developed tool for labeling excitatory or inhibitory synapses, with the benefit of not altering endogenous synaptic protein expression levels or synaptic transmission. Here, we generated a viral vector FingR toolbox that allows for multi-color, neuron-type-specific labeling of excitatory or inhibitory synapses in multiple brain regions. We screened various fluorophores, FingR fusion configurations, and transcriptional control regulations in adeno-associated virus (AAV) and retrovirus vector designs. We report the development of a red FingR variant and demonstrated dual labeling of excitatory and inhibitory synapses in the same cells. Furthermore, we developed cre-inducible FingR AAV variants and demonstrated their utility, finding that the density of inhibitory synapses in aspiny striatal cholinergic interneurons remained unchanged in response to dopamine depletion. Finally, we generated FingR retroviral vectors, which enabled us to track the development of excitatory and inhibitory synapses in hippocampal adult-born granule cells.

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Citations

Nov 6, 2020·Current Protocols in Neuroscience·James S Trimmer
Jun 25, 2021·Chemical Society Reviews·Golnaz KamaliniaRichard W Roberts

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Datasets Mentioned

BETA
MT586119
MT612428
MT612429
MT612430
MT612431
MT612432

Methods Mentioned

BETA
transgenic
transfection
electron microscopy
confocal

Software Mentioned

FingR

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