A Virus Infecting Marine Photoheterotrophic Alphaproteobacteria (Citromicrobium spp.) Defines a New Lineage of ssDNA Viruses

Frontiers in Microbiology
Qiang ZhengNianzhi Jiao

Abstract

In recent metagenomic studies, single-stranded DNA (ssDNA) viruses that infect bacteria have been shown to be diverse and prevalent in the ocean; however, there are few isolates of marine ssDNA phages. Here, we report on a cultivated ssDNA phage (vB_Cib_ssDNA_P1) that infects Citromicrobium bathyomarinum RCC1878 (family Sphingomonadaceae), and other members of the genus. This is the first ssDNA phage reported to infect marine alphaproteobacteria, and represents a newly recognized lineage of the Microviridae infecting members of Sphingomonadaceae, the Amoyvirinae. The ∼26 nm diameter polyhedral capsid contains a 4,360 bp genome with 6 open reading frames (ORFs) and a 59.3% G+C content. ORF1 encodes the capsid protein and ORF3 encodes the replication initiator protein. The replication cycle is ∼5 h, followed by a burst releasing about 180 infectious particles. The closest relative of vB_Cib_ssDNA_P1 is a prophage within the genome of Novosphingobium tardaugens strain NBRC16725. Phylogenetic analysis indicates that the vB_Cib_ssDNA_P1 phage and two related prophages, as well as an environmental sequence, form a novel group within the Microviridae. Our results indicate that this is a previously unknown lineage of ssDNA viruses whic...Continue Reading

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Citations

Jan 8, 2020·Mucosal Immunology·R SaussetM De Paepe
Feb 27, 2020·ELife·Paul C Kirchberger, Howard Ochman
Feb 23, 2021·Integrative Biology : Quantitative Biosciences From Nano to Macro·Tianyi Jin, John Yin
Jun 3, 2021·Microorganisms·Kathryn ForconeCynthia B Silveira

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Velvet
MASCOT
BLASTP
RAxML
ORF Finder
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BLAST
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