A virus-like particle vaccine for epidemic Chikungunya virus protects nonhuman primates against infection.

Nature Medicine
Wataru AkahataGary J Nabel

Abstract

Chikungunya virus (CHIKV) has infected millions of people in Africa, Europe and Asia since this alphavirus reemerged from Kenya in 2004. The severity of the disease and the spread of this epidemic virus present a serious public health threat in the absence of vaccines or antiviral therapies. Here, we describe a new vaccine that protects against CHIKV infection of nonhuman primates. We show that selective expression of viral structural proteins gives rise to virus-like particles (VLPs) in vitro that resemble replication-competent alphaviruses. Immunization with these VLPs elicited neutralizing antibodies against envelope proteins from alternative CHIKV strains. Monkeys immunized with VLPs produced high-titer neutralizing antibodies that protected against viremia after high-dose challenge. We transferred these antibodies into immunodeficient mice, where they protected against subsequent lethal CHIKV challenge, indicating a humoral mechanism of protection. Immunization with alphavirus VLP vaccines represents a strategy to contain the spread of CHIKV and related pathogenic viruses in humans.

References

Apr 1, 1990·The Journal of General Virology·M O McClureR A Weiss
Nov 26, 1993·Science·M F BachmannR M Zinkernagel
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·L NaldiniI M Verma
Mar 14, 1998·The Journal of Infectious Diseases·D J McClainF J Malinoski
Dec 22, 1999·Journal of Structural Biology·S J LudtkeW Chiu
Apr 17, 2001·The American Journal of Tropical Medicine and Hygiene·R EdelmanJ A Mangiafico
Oct 22, 2002·Journal of Virology·Wei ZhangMichael G Rossmann
Sep 19, 2003·Trends in Microbiology·Rob Noad, Polly Roy
Jan 1, 1962·Cold Spring Harbor Symposia on Quantitative Biology·D L CASPAR, A KLUG
Jul 25, 2006·Journal of Structural Biology·Guang TangSteven J Ludtke
Jun 5, 2007·Expert Review of Vaccines·Bryce Chackerian
Jul 3, 2007·PLoS Pathogens·Marion SourisseauOlivier Schwartz
Aug 19, 2007·The Journal of General Virology·Ann M Powers, Christopher H Logue
Dec 12, 2007·PLoS Pathogens·Konstantin A TsetsarkinStephen Higgs
Dec 18, 2007·Current Opinion in Biotechnology·Christine Ludwig, Ralf Wagner
May 13, 2008·Vaccine·Karuppiah MuthumaniDavid B Weiner
May 20, 2008·Science·Martin Enserink
Aug 12, 2008·Vaccine·Eryu WangScott C Weaver
Dec 9, 2008·The Medical Clinics of North America·Fabrice SimonPhilippe Parola
Jul 4, 2009·The Journal of Infectious Diseases·Thérèse CoudercMarc Lecuit
Aug 12, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J Erin StaplesAnn M Powers

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Citations

Jan 22, 2013·Archives of Virology·Jyoti RanaSanjay Gupta
Sep 25, 2012·Molecular Biotechnology·Andris Zeltins
Jun 1, 2010·Indian Journal of Virology : an Official Organ of Indian Virological Society·C V M Naresh Kumar, D V R Sai Gopal
Jul 3, 2013·Antiviral Research·Simon-Djamel ThibervilleXavier de Lamballerie
Feb 9, 2011·Immunology and Cell Biology·Alessandra Mortellaro, Paola Ricciardi-Castagnoli
Oct 28, 2010·Current Opinion in HIV and AIDS·Georgia D Tomaras, Barton F Haynes
Jan 11, 2013·Open Biology·Atanas Patronov, Irini Doytchinova
Feb 15, 2013·Journal of Clinical Microbiology·Natsuko KishishitaSurapee Anantapreecha
May 31, 2013·Journal of Virology·Jonathan E SnyderRichard J Kuhn
Oct 19, 2012·Journal of Virology·Anasuya ChattopadhyayJohn K Rose
Jun 4, 2010·Journal of Virology·Joy GardnerAndreas Suhrbier
Dec 12, 2012·Journal of Virology·Svetlana AtashevaIlya Frolov
Jul 21, 2011·Journal of Drug Delivery·Aiala SalvadorJosé Luis Pedraz
Feb 25, 2010·The Journal of Clinical Investigation·Stephen Higgs, Sarah A Ziegler
Dec 6, 2012·Virology Journal·Anuj SharmaRadha K Maheshwari
Jan 26, 2011·PLoS Neglected Tropical Diseases·Karthik MallilankaramanDavid B Weiner
Nov 8, 2012·PLoS Neglected Tropical Diseases·Andrew F van den HurkScott L O'Neill
Mar 22, 2013·PLoS Neglected Tropical Diseases·Stefan W MetzGorben P Pijlman
Sep 27, 2013·PLoS Neglected Tropical Diseases·Suganya SelvarajahGraham Simmons
Jul 25, 2014·PLoS Neglected Tropical Diseases·James Weger-LucarelliJorge E Osorio
Apr 16, 2011·PloS One·Pramila WalpitaLinfa Wang
Sep 24, 2013·BioMed Research International·Giuseppe SauttoRoberto Burioni
Apr 10, 2012·Annual Review of Biochemistry·Nicola G A AbresciaDavid I Stuart
Jun 24, 2014·BioMed Research International·Deepti Parashar, Sarah Cherian

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Datasets Mentioned

BETA
EU224270

Methods Mentioned

BETA
transfection
electron microscopy
PCR

Software Mentioned

EMAN
e2boxer
Prism
EMAN2
CTFIT

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