A visible fluorescent nanovaccine based on functional genipin crosslinked ovalbumin protein nanoparticles

Nanomedicine : Nanotechnology, Biology, and Medicine
Xia DongFeng Lv

Abstract

Accurate and efficient antigen delivery is crucial for inducing a strong and long-term immune response. A visible protein nanovaccine made from antigen could provide a novel and promising technology for secure and efficient delivery of the antigen with imaging visualization. In this study, a functional nanovaccine based on genipin crosslinked ovalbumin (OVA) fluorescent nanoparticles with chitosan (CS-OVA-NPs) was developed. The nanovaccine can carry abundant antigens by self-crosslinking without additional carriers. The fluorescence imaging technique was applied to monitor and reveal the process of antigen delivery in vivo based on the fluorescence of genipin with a non-invasive and real-time manner. This functional OVA nanovaccine can enhance the uptake of OVA in Dendritic Cells (DCs) and further promote DCs to maturate in vitro. In vivo study further indicated CS-OVA-NPs could trigger antigen-specific immune responses, which demonstrated that this fluorescent nanovaccine provided a novel design approach for accurate and efficient vaccine delivery.

Citations

Jul 31, 2018·Journal of Biophotonics·Edorta Santos-VizcainoJose Luis Pedraz
Jan 23, 2019·Advanced Healthcare Materials·Eun Sook LeeJae Hyung Park
Sep 29, 2020·Expert Opinion on Drug Delivery·Aranit HarizajKevin Braeckmans
May 30, 2021·Advanced Materials·DaeYong LeeBetty Y S Kim

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