A visual thalamocortical slice

Nature Methods
Jason N MacLeanRafael Yuste

Abstract

We describe a thalamocortical slice preparation in which connectivity between the mouse lateral geniculate nucleus (LGN) and primary visual cortex (V1) is preserved. Through DiI injections in fixed brains we traced and created a three-dimensional model of the mouse visual pathways. From this computer model we designed a slice preparation that contains a projection from LGN to V1. We prepared brain slices with these predicted coordinates and demonstrated anatomical LGN-V1 connectivity in these slices after LGN tracer injections. We also revealed functional LGN-V1 connectivity by stimulating LGN electrically and detecting responses in layer 4 of V1 using calcium imaging, field potential recordings and whole-cell recordings. We also identified layer-4 neurons that receive direct thalamocortical input. Finally, we compared cortical activity after LGN stimulation with spontaneous cortical activity and found significant overlap of the spatiotemporal dynamics generated by both types of events.

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Citations

Feb 25, 2014·Neurophysiology·Charles C Lee, Kazuo Imaizumi
Feb 15, 2011·Nature Methods·Yoan LeChasseurYves De Koninck
Dec 8, 2009·Nature Neuroscience·Brian B TheyelS Murray Sherman
Jun 12, 2009·Cerebral Cortex·Marc A ParentLi-Lian Yuan
Dec 19, 2008·PloS One·Brendon O WatsonRafael Yuste
Nov 4, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jay A BlundonStanislav S Zakharenko
Sep 10, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jae-eun Kang MillerRafael Yuste
May 21, 2015·Proceedings of the National Academy of Sciences of the United States of America·Makoto MatsuyamaYasushi Miyashita
Sep 29, 2012·Journal of Neuroscience Methods·Lovisa Case, Christian Broberger
Dec 28, 2010·Hearing Research·Charles C Lee, S Murray Sherman
May 16, 2009·Journal of Neuroscience Methods·Komal RambaniSteve M Potter
Mar 3, 2007·Molecular and Cellular Neurosciences·Michael I DawJohn T R Isaac
Feb 20, 2007·Journal of Neuroscience Methods·Mary M Rocco, Joshua C Brumberg
Mar 22, 2014·Frontiers in Neural Circuits·Paul V WatkinsPatrick O Kanold
Nov 14, 2017·Journal of Neural Engineering·Renaud SchuckSimon R Schultz
Oct 10, 2012·The Journal of Endocrinology·Alberto LoizzoStefano Loizzo
Apr 6, 2007·Nature·Feng ZhangKarl Deisseroth
May 24, 2008·Journal of Neurophysiology·Sylvain RheimsYuri Zilberter
Oct 11, 2013·Journal of Neurophysiology·Daniel A LlanoKevin A Stebbings
Sep 8, 2019·Brain Structure & Function·Augustin WalterArmelle Rancillac
Jan 27, 2020·The Journal of Comparative Neurology·Akihisa Kimura
Mar 17, 2017·Annual Review of Biophysics·Luis Carrillo-ReidRafael Yuste

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