A voltage-dependent porin-like channel in the inner envelope membrane of plant chloroplasts.

The Journal of Biological Chemistry
B Fuks, F Homblé

Abstract

The electrical activity of a single channel of 525 +/- 12 picosiemens in 150 mM KCl was measured after fusion of the inner envelope membrane of the chloroplast with planar lipid bilayers. The reversal potentials measured in KCl gradients indicate that this channel is weakly anion selective (PCl/PK = 1.6 +/- 0.2). The gating mechanism of the pore is voltage dependent. The channel shifts from a fully open state to a substrate at positive electrical potentials and remains closed at negative electrical potentials. Succinylation of the protein increases the open probability of the fully open state and reverses the channel selectivity. Analysis of the single-channel conductance as a function of the salt concentration and of the open probability at various voltages suggests that this channel is a new membrane porin not previously identified.

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Citations

Oct 1, 1996·Plant Physiology·B Fuks, F Homblé
Jul 31, 2013·Biophysical Chemistry·Vanessa ChecchettoIldiko Szabo
Jan 13, 2006·Current Biology : CB·Elizabeth S Haswell, Elliot M Meyerowitz
Dec 29, 2004·Biochimica Et Biophysica Acta·David G Mendoza-Cózatl, Rafael Moreno-Sánchez
Dec 31, 1997·The Journal of Biological Chemistry·P W van den Wijngaard, W J Vredenberg
Nov 29, 2005·Journal of Chemical Information and Modeling·Anna SegallaGiorgio Mario Giacometti

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