A voltage-dependent role for K+ in recovery from C-type inactivation

Biophysical Journal
D I Levy, C Deutsch

Abstract

Recovery from C-type inactivation of Kv1.3 can be accelerated by the binding of extracellular potassium to the channel in a voltage-dependent fashion. Whole-cell patch-clamp recordings of human T lymphocytes show that Ko+ can bind to open or inactivated channels. Recovery is biphasic with time constants that depend on the holding potential. Recovery is also dependent on the voltage of the depolarizing pulse that induces the inactivation, consistent with a modulatory binding site for K+ located at an effective membrane electrical field distance of 30%. This K(+)-enhanced recovery can be further potentiated by the binding of extracellular tetraethylammonium to the inactivated channel, although the tetraethylammonium does not interact directly with the K(+)-binding site. Our findings are consistent with a model in which K+ can bind and unbind slowly from a channel in the inactivated state, and inactivated channels that are bound by K+ will recover with a rate that is fast relative to unbound channels. Our data suggest that the kinetics of K+ binding to the modulatory site are slower than these recovery rates, especially at hyperpolarized voltages.

Citations

Jan 24, 2007·Pflügers Archiv : European journal of physiology·Igor I PottosinOxana R Dobrovinskaya
Nov 14, 1997·Neuron·M S Siegel, E Y Isacoff
Jan 13, 1998·Current Opinion in Biotechnology·M D Cahalan, K G Chandy
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·Z M PeiJ I Schroeder
Jul 1, 1997·The Journal of General Physiology·C TownsendR Horn
Aug 1, 1997·The Journal of General Physiology·G Yellen
Aug 1, 1997·The Journal of General Physiology·F S ChenD Fedida
Feb 2, 1999·The Journal of General Physiology·C Townsend, R Horn
Nov 7, 2001·The Journal of General Physiology·T LuJ Yang
Jul 19, 2006·The Journal of General Physiology·Evan C Ray, Carol Deutsch
May 28, 2013·Pflügers Archiv : European journal of physiology·Yen May ChengSteven J Kehl
Apr 16, 2014·Proceedings of the National Academy of Sciences of the United States of America·Andrew S ThomsonBrad S Rothberg
Nov 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Shian LiuSteve W Lockless
Jul 16, 2005·The Journal of Physiology·Harley T KurataDavid Fedida
Oct 1, 2011·Biochimica Et Biophysica Acta·Jason G McCoy, Crina M Nimigean
Jul 21, 2001·Biophysical Journal·K G KlemicS W Jones
Oct 24, 2007·Biophysical Journal·Yu A KaulinM Covarrubias
Jul 2, 1999·Biophysical Journal·D Kerschensteiner, M Stocker
Jan 28, 2003·Biophysical Journal·Mohammad Shahidullah, Manuel Covarrubias
Jan 31, 2012·Journal of Theoretical Biology·Geoffrey V MartinLaura Conforti
Jun 22, 2007·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Xianpei WangDanna Tu
Dec 25, 2003·The Journal of Physiology·Julien HeringRégis C Lambert
Jul 22, 2004·Journal of Neurobiology·Mark FryFrances Moody-Corbett
May 17, 2013·American Journal of Physiology. Renal Physiology·Gustavo FrindtLawrence G Palmer
Jun 18, 2004·American Journal of Physiology. Cell Physiology·Sándor SomodiGyörgy Panyi
May 23, 2020·The Journal of General Physiology·Tibor G SzantoGyorgy Panyi
May 21, 2021·The Journal of General Physiology·Tibor G SzantoGyorgy Panyi

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