PMID: 8944632Nov 1, 1996Paper

A voltage-operable current is involved in Ca2+ entry in human lymphocytes whereas ICRAC has no apparent role

The American Journal of Physiology
J J DensmoreL S Gray

Abstract

Presently, it is thought that a non-voltage-gated current is responsible for activation-induced Ca2+ entry in nonelectrically excitable cells such as lymphocytes. However, it has also been proposed that the pathway instead involves a second messenger-regulated Ca2+ channel that is voltage operable, where "voltage operable" is defined as an intrinsic property of the channel protein(s) rather than a requirement of normal gating. To evaluate the contribution of these currents to activation-induced Ca2+ influx, each was examined with respect to its ability to account for Ca2+ influx as reported by Ca(2+)-sensitive dyes. We identified a set of reagents, nordihydroguaiaretic acid and various calmodulin inhibitors, that inhibits Ca2+ entry and blocks the voltage-operable current but leaves the non-voltage-gated current unaltered. Further-more, nordihydroguaiaretic acid inhibited Ca(2+)-dependent proliferation of mitogen-activated human peripheral blood mononuclear cells or Jurkat T cells and specifically blocked Ca(2+)-dependent interleukin 2 production by Jurkat T cells to a degree similar to the immunosuppressant drug cyclosporin A. We also identified compounds, amiloride and Mn2+, that block the non-voltage-gated current but have n...Continue Reading

References

Aug 1, 1988·The Journal of General Physiology·R Horn, A Marty
Jul 1, 1988·The Journal of Physiology·A PeresR Zippel
Jan 1, 1989·Methods in Cell Biology·F Di VirgilioS C Silverstein
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Jul 1, 1994·Biophysical Journal·A Lückhoff, D E Clapham
Jan 1, 1994·Annual Review of Biochemistry·G R Crabtree, N A Clipstone
Sep 1, 1993·Pflügers Archiv : European journal of physiology·E Donnadieu, A Trautmann
May 1, 1993·Pflügers Archiv : European journal of physiology·C FasolatoR Penner

❮ Previous
Next ❯

Citations

Dec 6, 2002·The Journal of Biological Chemistry·Carol A GilchristWilliam A Petri
Sep 5, 2003·The Journal of Biological Chemistry·Maya F KotturiWilfred A Jefferies
Jun 13, 1997·The Journal of Biological Chemistry·D M HaverstickL S Gray
Feb 21, 2007·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Eva CagideLuis M Botana
Jun 23, 2006·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·M Carmen LouzaoLuis M Botana
Jan 30, 1999·Cell Calcium·D M HaverstickL S Gray
Mar 21, 2008·European Journal of Pharmacology·Ingrid LajdovaAlzbeta Chorvatova

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.