A water-soluble fraction from adult bone stimulates the differentiation of cartilage in explants of embryonic muscle

Differentiation; Research in Biological Diversity
P A LucasA I Caplan

Abstract

A water-soluble fraction of a 4 M guanidine HCl extract of demineralized adult bovine bone stimulated the differentiation of cartilage in explants of minced skeletal muscle from embryonic chick legs; cartilage was also induced by a semipurified protein preparation. Cartilage could be identified in treated cultures at 1 week with muscle from day-9 embryos, not before 2 weeks with muscle from day-12 embryos, and not before 3 weeks with muscle from day-19 embryos. The ability to respond to this water-soluble fraction by exhibiting cartilage differentiation was dose-dependent, but not confined to any particular muscle region of the day-12 embryonic leg. These observations indicate that bone-derived soluble chondroinductive agents act on cells in minced embryonic muscle preparations. The induction of cartilage is dependent upon the accessibility of the responding cells to the agents, on the concentration of inductive agents, and on the developmental age of the responsive tissue.

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Citations

May 10, 2002·Journal of Biomedical Materials Research·Tun AungNorio Ohshima
Feb 1, 1997·Mund-, Kiefer- und Gesichtschirurgie : MKG·N R Kübler
Jan 27, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J E OnyiaJ M Hock
Sep 1, 1993·In Vitro Cellular & Developmental Biology. Animal·H E YoungP A Lucas
Apr 30, 1999·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·B D BoyanZ Schwartz
Sep 1, 1991·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A I Caplan
Jan 13, 2011·Veterinary Surgery : VS·Nakia D SpencerMandi J Lopez

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