A wheat-based diet enhances colonization with the intestinal spirochaete Brachyspira intermedia in experimentally infected laying hens

Avian Pathology : Journal of the W.V.P.A
Nyree D PhillipsD J Hampson

Abstract

This study investigated the influence of feeding diets based on cereals with different non-starch polysaccharide content on colonization with the intestinal spirochaete Brachyspira intermedia in experimentally infected laying hens. The diets were based on wheat, barley, or barley and sorghum, all with or without supplementation with exogenous dietary enzymes. Six groups of 12 individually caged laying hens were fed the respective diets for 2 weeks, and then challenged at 20 weeks of age by crop tube with 10(8) to 10(9) colony forming units of active motile spirochaetes on five successive days. Eggs were collected daily. The birds were weighed weekly, and caecal faeces collected for assessment of water content. Every 3 to 4 days cloacal swabs were collected and subjected to culture and subsequent polymerase chain reaction for B. intermedia. The birds were killed at 27 weeks of age. Caecal contents were cultured for spirochaetes, and the viscosity of the ileal and rectal contents assessed. Dietary soluble non-starch polysaccharide content was not a consistent predictor of viscosity in the ileum, and the addition of enzymes did not significantly reduce the viscosity. A dietary influence on colonization was observed, with birds fed...Continue Reading

References

Oct 1, 1986·Journal of Clinical Microbiology·R A KunkleJ M Kinyon
Oct 24, 1981·The Veterinary Record·S R Jenkinson, C R Wingar
Oct 1, 1996·Australian Veterinary Journal·A J McLarenS L Wylie
Sep 4, 1999·British Poultry Science·S T PetersenM R Bedford
Jun 1, 1998·Nutrition Research Reviews·M R Bedford, H Schulze
Oct 1, 1999·Avian Pathology : Journal of the W.V.P.A·C P Stephens, D J Hampson

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Citations

Mar 15, 2005·Avian Pathology : Journal of the W.V.P.A·Nyree D PhillipsDavid J Hampson
Dec 21, 2013·Veterinary Microbiology·Luke J MappleyMartin J Woodward

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

InStat

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