A whole-cell electron tomography model of vacuole biogenesis in Arabidopsis root cells

Nature Plants
Yong CuiLiwen Jiang

Abstract

Plant vacuoles are dynamic organelles that play essential roles in regulating growth and development. Two distinct models of vacuole biogenesis have been proposed: separate vacuoles are formed by the fusion of endosomes, or the single interconnected vacuole is derived from the endoplasmic reticulum. These two models are based on studies of two-dimensional (2D) transmission electron microscopy and 3D confocal imaging, respectively. Here, we performed 3D electron tomography at nanometre resolution to illustrate vacuole biogenesis in Arabidopsis root cells. The whole-cell electron tomography analysis first identified unique small vacuoles (SVs; 400-1,000 nm in diameter) as nascent vacuoles in early developmental cortical cells. These SVs contained intraluminal vesicles and were mainly derived/matured from multivesicular body (MVB) fusion. The whole-cell vacuole models and statistical analysis on wild-type root cells of different vacuole developmental stages demonstrated that central vacuoles were derived from MVB-to-SV transition and subsequent fusions of SVs. Further electron tomography analysis on mutants defective in MVB formation/maturation or vacuole fusion demonstrated that central vacuole formation required functional MVBs ...Continue Reading

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Citations

Aug 23, 2019·Proceedings of the National Academy of Sciences of the United States of America·Wenna ZhengYan Guo
Oct 2, 2019·Proceedings of the National Academy of Sciences of the United States of America·Cecilia Rodriguez-FurlanGlenn R Hicks
Aug 31, 2019·Protoplasma·Yong CuiLiwen Jiang
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Nov 11, 2019·Science China. Life Sciences·Dongmei ZhuJinbo Shen
Jun 18, 2020·International Journal of Molecular Sciences·Yiran CaoDabing Zhang
Nov 23, 2020·The Plant Journal : for Cell and Molecular Biology·Germán RobertKohki Yoshimoto
Oct 22, 2020·Journal of Integrative Plant Biology·Xueting WangYongliang Zhang
May 15, 2020·Trends in Plant Science·Yong CuiLiwen Jiang
Mar 3, 2021·ACS Nano·Carlos Sanchez-CanoWolfgang J Parak
Apr 2, 2021·Plant Physiology·Zhiqi LiuLiwen Jiang

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Methods Mentioned

BETA
transmission electron microscopy
confocal microscopy
confocal
electron microscopy
scanning electron microscopy
electron tomography
transgenic
GTPase
nucleotide exchange
transmission

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