A widely conserved bacterial cell division protein that promotes assembly of the tubulin-like protein FtsZ

Genes & Development
Frederico Gueiros-Filho, Richard Losick

Abstract

Cell division in bacteria is mediated by the tubulin-like protein FtsZ, which assembles into a structure known as the Z ring at the future site of cytokinesis. We report the discovery of a Z-ring-associated protein in Bacillus subtilis called ZapA. ZapA was found to colocalize with the Z ring in vivo and was capable of binding to FtsZ and stimulating the formation of higher-order assemblies of the cytokinetic protein in vitro. The absence of ZapA alone did not impair cell viability, but the absence of ZapA in combination with the absence of a second, dispensable division protein EzrA caused a severe block in cytokinesis. The absence of ZapA also caused lethality in cells producing lower than normal levels of FtsZ or lacking the division-site-selection protein DivIVA. Conversely, overproduction of ZapA reversed the toxicity of excess levels of the division inhibitor MinD. In toto, the evidence indicates that ZapA is part of the cytokinetic machinery of the cell and acts by promoting Z-ring formation. Finally, ZapA is widely conserved among bacteria with apparent orthologs in many species, including Escherichia coli, in which the orthologous protein exhibited a strikingly similar pattern of subcellular localization to that of Zap...Continue Reading

References

Jan 1, 1990·Research in Microbiology·C L WoldringhN Nanninga
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·P J YoungmanR Losick
Feb 10, 1995·Cell·H P Erickson
Feb 1, 1994·Current Opinion in Cell Biology·N Hirokawa
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·H P EricksonD Bramhill
Feb 28, 1998·The EMBO Journal·A Mukherjee, J Lutkenhaus
Apr 21, 1999·Molecular Microbiology·Z LiuJ Lutkenhaus
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·D M Raskin, P A de Boer
May 6, 1999·The EMBO Journal·J Löwe, L A Amos
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·P A LevinA D Grossman
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·Z HuJ Lutkenhaus
Dec 30, 1999·Journal of Bacteriology·C LuH P Erickson
Jan 14, 2000·Journal of Bacteriology·S L RowlandL I Rothfield
Jan 21, 2001·The Journal of Biological Chemistry·L RombergH P Erickson
Jun 13, 2001·Molecular Microbiology·E J Harry
Aug 22, 2001·Journal of Bacteriology·P A LevinA D Grossman
Dec 4, 2001·Current Opinion in Microbiology·F van den EntJ Löwe
Dec 4, 2001·Current Opinion in Microbiology·W Margolin
Feb 16, 2002·The EMBO Journal·Sebastien Pichoff, Joe Lutkenhaus
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jesse StrickerHarold P Erickson

❮ Previous
Next ❯

Citations

Oct 7, 2006·Applied Microbiology and Biotechnology·Waldemar Vollmer
Aug 31, 2012·Cell Biochemistry and Biophysics·Weiling HongJianping Xie
Mar 24, 2004·Current Opinion in Cell Biology·Linda A AmosJan Löwe
Dec 16, 2003·Journal of Molecular Biology·Martin Howard
Dec 10, 2003·Current Opinion in Microbiology·Klas Flärdh
Oct 24, 2003·Trends in Cell Biology·Rut Carballido-López, Jeff Errington
Aug 6, 2003·FEMS Microbiology Letters·Elizabeth TenorioKatsumi Isono
Mar 8, 2008·Nature Reviews. Drug Discovery·Rowena L Lock, Elizabeth J Harry
Oct 18, 2005·Nature Reviews. Molecular Cell Biology·William Margolin
Aug 15, 2009·Nature Reviews. Microbiology·David W Adams, Jeff Errington
Aug 12, 2006·Physical Chemistry Chemical Physics : PCCP·Alison RodgerTimothy R Dafforn
Oct 19, 2011·Proceedings of the National Academy of Sciences of the United States of America·Desirée C YangThomas G Bernhardt
Jan 6, 2011·Genes & Development·Joost WillemseGilles P van Wezel
Mar 3, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Stephen G AddinallDavid I Roper
Sep 9, 2008·Journal of Bacteriology·José Roberto TavaresFrederico J Gueiros-Filho
Apr 17, 2012·Journal of Bacteriology·Jorge Durand-HerediaAnuradha Janakiraman
Jun 26, 2012·Journal of Bacteriology·Jennifer K Wagner-HermanDavid Z Rudner
Oct 4, 2006·Journal of Bacteriology·Masaki Osawa, Harold P Erickson
Nov 14, 2006·Journal of Bacteriology·Daniela FaddaOrietta Massidda
Jan 11, 2011·Journal of Bacteriology·Jorge M Durand-HerediaAnuradha Janakiraman
Oct 30, 2012·Journal of Bacteriology·I C SilvaH Ferreira
Feb 20, 2007·Journal of Bacteriology·Brian D CorbinWilliam Margolin
May 12, 2009·Journal of Bacteriology·Pamela GambaRichard A Daniel
Mar 5, 2013·Journal of Bacteriology·Kuo-Hsiang HuangAnuradha Janakiraman
Feb 19, 2013·Journal of Bacteriology·Katherine CellerGilles P van Wezel
Nov 8, 2011·Journal of Bacteriology·Elisa Galli, Kenn Gerdes
May 15, 2012·Journal of Bacteriology·Valquiria Tiago dos SantosFrederico J Gueiros-Filho
Jun 19, 2004·Journal of Bacteriology·Karen CarniolRichard Losick
Aug 20, 2004·Journal of Bacteriology·Kuei-Min ChungBan-Yang Chang
Sep 3, 2004·Journal of Bacteriology·André PietteMartine Nguyen-Distèche
Jan 20, 2004·Journal of Bacteriology·Kari L SchmidtDavid S Weiss
Jan 20, 2004·Journal of Bacteriology·S J Ryan Arends, David S Weiss
Apr 20, 2005·Journal of Bacteriology·Nina GrantcharovaKlas Flärdh
Dec 15, 2005·Journal of Bacteriology·Miguel VicenteJesús Mingorance

❮ Previous
Next ❯

Related Concepts