A Wilms tumor cell line, HFWT, can greatly stimulate proliferation of CD56+ human natural killer cells and their novel precursors in blood mononuclear cells

Experimental Hematology
Hideki HaradaTadao Ohno

Abstract

A Wilms tumor cell line, HFWT, selectively stimulates expansion of natural killer (NK) cells from human peripheral blood mononuclear cells (PBMC). In this study, we attempted to identify NK precursors in PBMC or in cord blood mononuclear cells (CBMC) that preferentially respond to feeder HFWT cells. Human NK cells or candidate precursor cells were fractionated from PBMC or CBMC by magnetic antibody cell sorting or by flow cytometry and applied to limiting dilution analysis to determine the proportion of NK/NK precursor cells, which are able to proliferate on irradiated HFWT cells. NK and NK precursor cells were cultured in medium containing interleukin-2 (IL-2). Expansion of NK cells from both resting NK cells and NK precursor cells was examined using proliferation from single cells, expression of NK cell markers, and cytotoxic activity. In the limiting dilution analysis, NK cells expanded on irradiated HFWT cells not only from CD3-CD56bright and CD3-CD56dim NK cells, but also from CD16+/-CD122+ cells in the lineage-negative (Lin-, CD3-CD14-CD19-CD56-) cell fraction. The feeder HFWT cells stimulated Lin-CD122+ cell proliferation more strongly than feeder cells from the well-known human NK target cell line K562. CBMC contained s...Continue Reading

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Citations

Dec 10, 2013·Hematology·Richard W Childs, Maria Berg
Oct 3, 2009·British Journal of Haematology·Michael R Verneris, Jeffrey S Miller
Nov 15, 2011·Tissue Antigens·D R Shook, D Campana
Jan 13, 2015·Hematology/oncology and Stem Cell Therapy·Arundhati Mandal, Chandra Viswanathan
Sep 26, 2016·Blood Reviews·Geoffrey S KannanDean A Lee
May 5, 2017·International Reviews of Immunology·Senthilkumar PreethySamuel J K Abraham
Nov 24, 2005·European Journal of Immunology·Hideki HaradaSeiji Okada
May 12, 2017·Frontiers in Immunology·Markus GranzinEvelyn Ullrich
Dec 3, 2017·International Journal of Hematology·Catharina H M J Van Elssen, Stefan O Ciurea
Jan 8, 2020·Nature Reviews. Drug Discovery·Noriko ShimasakiDario Campana
Mar 27, 2021·Stem Cell Research & Therapy·Margaret G LambDean A Lee
Apr 7, 2021·Expert Review of Clinical Immunology·Kajal ChaudhryCatherine M Bollard

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