A Wolbachia triple-strain infection generates self-incompatibility in Aedes albopictus and transmission instability in Aedes aegypti.

Parasites & Vectors
Thomas H Ant, Steven P Sinkins

Abstract

Artificially-introduced transinfections of the intracellular bacterium Wolbachia pipientis have the potential to reduce the vectorial capacity of mosquito populations for viruses such as dengue and chikungunya. Aedes albopictus has two native strains of Wolbachia, but their replacement with the non-native wMel strain blocks transmission of both viruses. The pattern of cytoplasmic incompatiiblity generated by wMel with wild-types is bidirectional. Novel-plus-native-strain co-infection is predicted to lead to a more efficient population spread capacity; from a bi-directional to a uni-directional cytoplasmic incompatibility (CI) model. A novel-plus-native-strain triple-infection in Ae. albopictus (wAlbAwAlbBwMel) was generated. Although triple-infected females were fully reproductively viable with uninfected males, they displayed self-incompatibility. qPCR of specific strains in dissected tissues suggested that this may be due to the displacement of one of the native strains (wAlbA) from the ovaries of triple-infected females. When the triple strain infection was transferred into Aedes aegypti it displayed an unexpectedly low level of transmission fidelity of the three strains in this species. These results suggest that combining ...Continue Reading

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Citations

Apr 20, 2019·PLoS Neglected Tropical Diseases·Perran A RossAry A Hoffmann
Mar 11, 2020·PLoS Neglected Tropical Diseases·Maria Vittoria ManciniSteven P Sinkins
Sep 26, 2020·ELife·J Dylan ShropshireSeth R Bordenstein
Jun 2, 2020·Frontiers in Genetics·Rupert MazzuccoChristian Schlötterer
Sep 11, 2019·Annual Review of Genetics·Perran A RossAry A Hoffmann
Aug 10, 2020·Microbial Ecology·Panupong ThongpremGregory D D Hurst
Dec 29, 2020·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Noor Afizah AhmadSteven P Sinkins
Feb 16, 2021·Frontiers in Microbiology·Francesca ScolariMariangela Bonizzoni
May 1, 2021·Pathogens·Thiago Nunes PereiraLuciano Andrade Moreira
May 9, 2021·Parasites & Vectors·Ranju Ravindran Santhakumari ManojDomenico Otranto
Jul 26, 2021·Trends in Parasitology·Eric P CaragataLuciano A Moreira
Jul 21, 2021·Nature Communications·Guan-Hong WangOmar S Akbari
Sep 2, 2021·Microbial Genomics·Kimberley R DaintyHeather A Flores

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Methods Mentioned

BETA
transinfection
PCR

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