A WRKY transcription factor PbrWRKY53 from Pyrus betulaefolia is involved in drought tolerance and AsA accumulation

Plant Biotechnology Journal
Yue LiuXiaosan Huang

Abstract

WRKY comprises a large family of transcription factors in plants, but most WRKY members are still poorly understood. In this study, we report the identification and functional characterization of PbrWRKY53 isolated from Pyrus betulaefolia. PbrWRKY53 was greatly up-regulated by drought and abscisic acid, but slightly induced by salt and cold. Subcellar localization analyses showed that PbrWRKY53 was located in the nucleus. Ectopic expression of PbrWRKY53 in tobacco and Pyrus ussuriensis conferred enhanced tolerance to drought stress. The transgenic plants exhibited better water status, less reactive oxygen species generation and higher levels of antioxidant enzyme activities and metabolites than the wild type. In addition, overexpression of PbrWRKY53 in transgenic tobacco resulted in enhanced expression level of PbrNCED1, and led to the increase in larger amount of vitamin C accumulation in comparison to WT. Knock-down of PbrWRKY53 in P. ussuriensis down-regulated PbrNCED1 abundance, accompanied by compromised drought tolerance. Yeast one-hybrid assay, EMSA and transient expression analysis demonstrated that PbrWRKY53 could bind to the W-box element in the promoter region of PbrNCED1. Taken together, these results demonstrated t...Continue Reading

References

Apr 29, 2000·Trends in Plant Science·T EulgemI E Somssich
Jan 2, 2003·Annals of Botany·Olga BlokhinaKurt V Fagerstedt
Sep 13, 2003·Plant Physiology·Liming Xiong, Jian-Kang Zhu
Jan 5, 2005·BMC Evolutionary Biology·Yuanji Zhang, Liangjiang Wang
May 3, 2005·Annual Review of Plant Biology·Bob B Buchanan, Yves Balmer
Jul 24, 2007·Current Opinion in Plant Biology·Thomas Eulgem, Imre E Somssich
Aug 28, 2007·Nature Methods·Dmitry ShcherboDmitriy M Chudakov
Mar 6, 2008·BMC Genomics·Michaela Hundertmark, Dirk K Hincha
Jun 17, 2009·Molecular Plant·Andrea PitzschkeHeribert Hirt
Aug 29, 2009·Plant, Cell & Environment·Gad MillerRon Mittler
Mar 23, 2010·Trends in Plant Science·Paul J RushtonQingxi J Shen
May 22, 2010·The Plant Journal : for Cell and Molecular Biology·Xiaozhi RenZhizhong Gong
Sep 28, 2010·Plant Physiology and Biochemistry : PPB·Sarvajeet Singh Gill, Narendra Tuteja
Mar 19, 2011·Journal of Plant Research·Yasunari FujitaKazuko Yamaguchi-Shinozaki
Apr 14, 2011·Plant, Cell & Environment·Nobuhiro SuzukiGad Miller
Aug 30, 2012·Molecular Plant·Yanjuan JiangDiqiu Yu
Dec 10, 2013·Annual Review of Plant Biology·Honghong Hu, Lizhong Xiong
Dec 17, 2014·Plant Physiology·Craig Schluttenhofer, Ling Yuan

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Citations

Dec 19, 2019·Antioxidants·Karolina BilskaEwa Marzena Kalemba
Jan 26, 2021·Frontiers in Plant Science·Tao YaoJin-Gui Chen

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Methods Mentioned

BETA
transgenic
environmental stresses
AsA
MDA
Y1H
PCR
one‐hybrid
Assay

Software Mentioned

MAGA
ExPASy ( Expert Protein Analysis System
SPSS
Clustal W
Genedoc

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