PMID: 8593611Oct 1, 1995Paper

A YAC-based contig of 1.5 Mb spanning the human multidrug resistance gene region and delineating the amplification unit in three human multidrug-resistant cell lines

Genome Research
K TorigoeM Wada

Abstract

A contig of 21 nonchimeric yeast artificial chromosomes (YACs) has been assembled across 1.5 Mb of the multidrug resistance (MDR) gene region located at 7q21, and formatted with four previously reported probes, six newly isolated probes, and three sequence-tagged sites (STSs) from internal and end fragments of YACs. A physical map of rare cutter restriction enzyme sites across the region was also constructed by pulsed-field gel electrophoretic (PFGE) analysis of four overlapping YAC clones. The amplification unit of this region in different cell lines was then determined by Southern blot analysis on the basis of the physical map and probes. Amplified DNA was located in extrachromosomal elements in human MDR cell lines studied here, and the size of the amplification unit was determined to be discrete in one MDR amplification but variable in others.

References

Jan 1, 1992·Annual Review of Cell Biology·C F Higgins
Jan 1, 1992·Mammalian Genome : Official Journal of the International Mammalian Genome Society·S W SchererL C Tsui
Jan 1, 1991·DNA Sequence : the Journal of DNA Sequencing and Mapping·J A EndicottV Ling
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·E D Green, M V Olson
Dec 29, 1989·Biochemical and Biophysical Research Communications·K KohnoM Kuwano
Jan 1, 1989·Advances in Cancer Research·A M van der Bliek, P Borst
Sep 29, 1989·Science·M OlsonD Botstein
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C R FairchildK H Cowan
Nov 30, 1988·Gene·A M van der BliekP Borst
Nov 1, 1988·Japanese Journal of Cancer Research : Gann·K KohnoM Kuwano
Jul 1, 1986·Somatic Cell and Molecular Genetics·A FojoI Pastan
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P GrosD E Housman
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·I B RoninsonI Pastan
May 28, 1987·The New England Journal of Medicine·I Pastan, M Gottesman
Mar 1, 1985·Somatic Cell and Molecular Genetics·S AkiyamaM M Gottesman
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A T FojoI Pastan
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Jan 1, 1984·Annual Review of Biochemistry·G R Stark, G M Wahl
Mar 30, 1994·Biochemical and Biophysical Research Communications·K AsakunoM Kuwano

❮ Previous
Next ❯

Citations

May 8, 2000·International Journal of Cancer. Journal International Du Cancer·T HaradaM Wada
Jan 1, 1997·Genome Research·G G BouffardE D Green
Jan 12, 2021·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Luciana MoscaGianni Colotti

❮ Previous
Next ❯

Related Concepts

Related Feeds

Artificial Chromosomes

Artificial chromosomes are genetically engineered chromosomes derived from the DNA of a species. Discover the latest research on artificial chromosomes here.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Allen Institute for Brain Science Network

The Allen Institute for Brain Science Network is a not-for-profit biomedical research organization that provides open access to multiple neuroscience tools and resources. Find the latest research from tjr Allen Institute for Brain Science Network here.