A YAC contig joining the desmocollin and desmoglein loci on human chromosome 18 and ordering of the desmocollin genes

Genomics
C M CowleyR S Buxton

Abstract

The desmocollins and desmogleins are members of the cadherin family of adhesive proteins present in the desmosome type of cell-cell junction. All of the known desmoglein and desmocollin isoforms, which have differing tissue and developmental distributions, are coded by very closely linked genes at 18q12.1. We have previously described YAC clones carrying all three known desmoglein (DSG) genes. We have now isolated YAC clones that carry all three known desmocollin genes (DSC1, 2, and 3) from two libraries and also isolated clones that join the DSC locus to the DSG locus, forming a complete contig for the region. Absence of chimeric ends for some of the YACs was confirmed by isolating Vectorette PCR products for the YAC ends and mapping the derived DNA sequences back to other YACs from CEPH. The whole DSC/DSG gene complex occupies no more than about 700 kb, and the genes are arranged in the order cen-3'-DSC3-DSC2-DSC1-5'-5'-DSG1-DSG3-D SG2-3'-tel, so that the two gene clusters are transcribed outward from the interlocus region. A P1 clone carrying part of DSC2 and DSC3 confirmed the relative orientation of transcription of these two genes. The conservation of close genetic linkage may be of trivial importance related to the recen...Continue Reading

References

Jun 1, 1992·Seminars in Cell Biology·R S Buxton, A I Magee
Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·B C SorkinB A Cunningham
Aug 25, 1991·Nucleic Acids Research·M HattaM Takeichi
Oct 1, 1991·Current Opinion in Cell Biology·A I Magee, R S Buxton
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·Z LarinH Lehrach
Jan 1, 1990·Annual Review of Cell Biology·M A SchwarzW W Franke
Jun 1, 1988·Differentiation; Research in Biological Diversity·A MansouriR Kemler
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Nov 1, 1984·The Journal of Experimental Medicine·L KouluJ R Stanley
Jan 1, 1993·Journal of Cell Science. Supplement·E Fuchs
Oct 1, 1995·The British Journal of Dermatology·M DmochowskiD R Garrod
Mar 27, 1995·Annals of the New York Academy of Sciences·B D AngstA I Magee
Oct 1, 1994·Current Opinion in Cell Biology·P J Koch, W W Franke
Nov 1, 1994·The British Journal of Dermatology·M R JudgeJ I Harper
Sep 15, 1994·Biochemical and Biophysical Research Communications·M J BussemakersJ A Schalken
Jan 1, 1994·Cytogenetics and Cell Genetics·U FranckeP Wilgenbus
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·N S ShepherdN Sternberg
Mar 1, 1994·The Journal of Heredity·M T DavissonE M Eicher
Dec 22, 1995·Science·T J HudsonE S Lander
Jul 1, 1996·The Journal of Investigative Dermatology·X MontagutelliJ P Sundberg

❮ Previous
Next ❯

Citations

Apr 4, 2008·Histochemistry and Cell Biology·Jens Waschke
Mar 22, 2001·Experimental Dermatology·P B Cserhalmi-FriedmanA M Christiano
Sep 7, 2004·DNA Sequence : the Journal of DNA Sequencing and Mapping·Ana KljuicAngela M Christiano
Nov 12, 2013·Cell Communication & Adhesion·Robert M Harmon, Kathleen J Green
Mar 22, 2003·The Journal of Investigative Dermatology·Neil V Whittock, Christopher Bower
Jun 10, 2004·The Journal of Dermatology·Xing Cheng, Peter J Koch
Oct 23, 2012·Clinical and Experimental Dermatology·J V Oliveira JúniorV Aoki
Apr 12, 2005·European Journal of Cell Biology·Ansgar Schmidt, Stephanie Jäger
Dec 24, 1997·Differentiation; Research in Biological Diversity·I A KingR S Buxton
Jun 22, 1999·Differentiation; Research in Biological Diversity·A SchmidtW W Franke
Mar 13, 2003·Experimental Dermatology·A Kljuic, A M Christiano
Sep 23, 1998·Micron : the International Research and Review Journal for Microscopy·I D Burdett
Oct 12, 2000·Biochemical and Biophysical Research Communications·N V WhittockJ A McGrath
Feb 22, 2001·Biochemical and Biophysical Research Communications·P L GadhaviR S Buxton
Apr 28, 1999·Molecular Medicine Today·D P Kelsell, H P Stevens

❮ Previous
Next ❯

Related Concepts

Related Feeds

Artificial Chromosomes

Artificial chromosomes are genetically engineered chromosomes derived from the DNA of a species. Discover the latest research on artificial chromosomes here.