A Yeast Mutant Deleted of GPH1 Bears Defects in Lipid Metabolism

PloS One
Martina GsellGünther Daum

Abstract

In a previous study we demonstrated up-regulation of the yeast GPH1 gene under conditions of phosphatidylethanolamine (PE) depletion caused by deletion of the mitochondrial (M) phosphatidylserine decarboxylase 1 (PSD1) (Gsell et al., 2013, PLoS One. 8(10):e77380. doi: 10.1371/journal.pone.0077380). Gph1p has originally been identified as a glycogen phosphorylase catalyzing degradation of glycogen to glucose in the stationary growth phase of the yeast. Here we show that deletion of this gene also causes decreased levels of phosphatidylcholine (PC), triacylglycerols and steryl esters. Depletion of the two non-polar lipids in a Δgph1 strain leads to lack of lipid droplets, and decrease of the PC level results in instability of the plasma membrane. In vivo labeling experiments revealed that formation of PC via both pathways of biosynthesis, the cytidine diphosphate (CDP)-choline and the methylation route, is negatively affected by a Δgph1 mutation, although expression of genes involved is not down regulated. Altogether, Gph1p besides its function as a glycogen mobilizing enzyme appears to play a regulatory role in yeast lipid metabolism.

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Citations

Sep 14, 2016·Current Microbiology·Shilpa JainPeter Oelkers
Jul 8, 2020·Proceedings of the National Academy of Sciences of the United States of America·Morgan E CarterAdam J Bogdanove
Mar 14, 2021·Journal of Microbiological Methods·Micaele Rodrigues de SouzaHorllys Gomes Barreto

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Methods Mentioned

BETA
PCR
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fluorescence microscopy
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