A yeast two-hybrid study of human p97/Gab2 interactions with its SH2 domain-containing binding partners

FEBS Letters
Catherine CrouinJacques Bertoglio

Abstract

p97/Gab2 is a recently characterized member of a large family of scaffold proteins that play essential roles in signal transduction. Gab2 becomes tyrosine-phosphorylated in response to a variety of growth factors and forms multimolecular complexes with SH2 domain-containing signaling molecules such as the p85-regulatory subunit of the phosphoinositide-3-kinase (p85-PI3K), the tyrosine phosphatase SHP-2 and the adapter protein CrkL. To characterize the interactions between Gab2 and its SH2-containing binding partners, we designed a modified yeast two-hybrid system in which the Lyn tyrosine kinase is expressed in a regulated manner in yeast. Using this assay, we demonstrated that p97/Gab2 specifically interacts with the SH2 domains of PI3K, SHP-2 and CrkL. Interaction with p85-PI3K is mediated by tyrosine residues Y452, Y476 and Y584 of Gab2, while interaction with SHP-2 depends exclusively on tyrosine Y614. CrkL interaction is mediated by its SH2 domain recognizing Y266 and Y293, despite the latter being in a non-consensus (YTFK) environment.

References

Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J McGladeT Pawson
Mar 12, 1993·Cell·Z SongyangR J Lechleider
Jan 1, 1994·Journal of Cell Science. Supplement·L E Marengere, T Pawson
Nov 1, 1995·Trends in Biochemical Sciences·Z Songyang, L C Cantley
May 28, 1996·Proceedings of the National Academy of Sciences of the United States of America·K S RavichandranS J Burakoff
Jan 1, 1997·Annual Review of Biophysics and Biomolecular Structure·J Kuriyan, D Cowburn
Mar 10, 1998·Cell·P HofS E Shoelson
Oct 30, 1998·Journal of Clinical Immunology·F GesbertJ Bertoglio
Sep 13, 2000·Molecular and Cellular Biology·H GuB G Neel
Oct 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·J C PrattH Gu
Dec 16, 2000·Plant Molecular Biology·M A FreireC Robaglia

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Citations

Mar 12, 2003·Trends in Cell Biology·Haihua Gu, Benjamin G Neel
Mar 26, 2010·The Journal of Biological Chemistry·Emilia Alina BarbuReuben P Siraganian
Jul 21, 2012·The Journal of Biological Chemistry·Gaëlle CombeauJean-Vianney Barnier
Dec 12, 2012·Genes & Cancer·Emily S Bell, Morag Park
Mar 22, 2007·Blood·Yi ZhangKevin D Bunting
Sep 10, 2009·Cell Communication and Signaling : CCS·Franziska U WöhrleTilman Brummer
Aug 15, 2008·Breast Cancer Research : BCR·Christian D YoungSteven M Anderson
Apr 23, 2014·The Journal of Experimental Medicine·Lalit KumarRaif S Geha
Jan 5, 2012·Advances in Hematology·Sheetal VermaKevin D Bunting
Jun 23, 2015·Molecular Medicine Reports·Chen-Bo DingJun-Min Luo
Jan 7, 2009·The International Journal of Biochemistry & Cell Biology·Evan Ingley
Nov 17, 2009·Experimental Cell Research·Michael CholayLaurent Daviet
Dec 31, 2008·Molecular Membrane Biology·Leyuan BaoSreenivasan Ponnambalam
Oct 28, 2008·Cellular Signalling·Máté MausGabriella Sármay
Apr 12, 2002·FEBS Letters·Yan Liu, Larry R Rohrschneider
Jun 24, 2003·The Journal of Biological Chemistry·Chunmei ZhaoGen-Sheng Feng
Aug 13, 2020·International Journal of Molecular Sciences·Ji-Eun KimTae-Cheon Kang
Sep 10, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mary ArnaudJacques Bertoglio
Apr 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zhi-Hui XieReuben P Siraganian
Oct 20, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lulu CaoArthur R Salomon
Apr 9, 2021·Arteriosclerosis, Thrombosis, and Vascular Biology·Vijay KondreddyUsha R Pendurthi
Mar 21, 2003·Journal of Proteome Research·Daniel D Clark, Blake R Peterson

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