PMID: 9163323Apr 15, 1997Paper

A zinc-dependent DNA-binding activity co-operates with cAMP-responsive-element-binding protein to activate the human thyroglobulin enhancer

The Biochemical Journal
V BergD Christophe

Abstract

Footprinting experiments involving the human thyroglobulin gene enhancer and thyroid nuclear extracts revealed a protected region called X2, containing an incomplete cAMP-responsive element (CRE). Band-shift experiments identified two binding activities recognizing the X2 element: a CRE-binding protein (CREB)/activating transcription factor (ATF) relative that binds the half CRE motif and a second factor that interacts with a G-rich motif located just upstream from the CRE. The first factor appears to be CREB itself, as indicated by the supershifting when using an antibody directed against CREB, and the second DNA-binding activity involved was shown to be zinc-dependent and exhibited an apparent molecular mass of 42-44 kDa in South-Western blotting experiments. This factor may represent a novel entity, which we named CAF, for 'CREB Associated Factor'. Three copies of X2 sequence conferred a strong cAMP-dependent transcriptional activation to a heterologous promoter in transient transfection assay in cAMP-stimulated primary thyrocytes and HeLa cells. Transfection experiments of constructs containing the X2 element mutated in either the CRE or the G-rich site showed that both motifs were required for this transcription activating...Continue Reading

Citations

May 30, 2002·Thyroid : Official Journal of the American Thyroid Association·Thomas L SchmittUlrich Loos
Nov 11, 2016·Thyroid : Official Journal of the American Thyroid Association·Risheng MaTerry F Davies
May 11, 2018·Thyroid : Official Journal of the American Thyroid Association·Panos G ZirosGerasimos P Sykiotis
Mar 20, 2019·Nature Reviews. Endocrinology·Cintia E CitterioPeter Arvan
Feb 11, 2010·Journal of Cell Science·Chantal ChristisIneke Braakman
Feb 12, 1998·Biochemical and Biophysical Research Communications·M Kobayashi, K Kawakami
Jul 28, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Carina M Rivolta, Héctor M Targovnik

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