A zinc finger motif-containing protein is essential for chloroplast RNA editing

PLoS Genetics
Tao SunMaureen R Hanson

Abstract

C-to-U editing of transcripts in plant organelles is carried out by small (<400 kD) protein complexes called editosomes. Recognition of the proper C target for editing is mediated by pentatricopeptide repeat (PPR) containing proteins that recognize cis-elements. Members of two additional gene families, the RIP/MORF and ORRM families, have each been found to be required for editing of particular sets of Cs in mitochondria and/or chloroplasts. By co-immunoprecipitation of the chloroplast editing factor ORRM1, followed by mass spectrometry, we have now identified a member of the RanBP2 type zinc fingers (pFAM00641) protein family that is required for editing of 14 sites in chloroplasts and affects editing efficiency of another 16 chloroplast C targets. In yeast two-hybrid assays, OZ1 (Organelle Zinc finger 1) interacts with PPR site recognition factors whose cognate sites are affected when OZ1 is mutated. No interaction of OZ1 with the chloroplast editing factors RIP2 and RIP9 was detected; however, OZ1 interacts with ORRM1, which binds to RIP proteins, allowing us to build a model for the chloroplast RNA editosome. The RNA editosomes that act upon most chloroplast C targets are likely to contain a PPR protein recognition factor, ...Continue Reading

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Citations

Dec 28, 2016·Genes·Mizuho Ichinose, Mamoru Sugita
May 27, 2017·Journal of Experimental Botany·Xiaowen ShiStéphane Bentolila
Nov 8, 2017·PloS One·Nuria Andrés-Colás, Dominique Van Der Straeten
Aug 2, 2017·Proceedings of the National Academy of Sciences of the United States of America·Damien GuillaumotClaire Lurin
Apr 17, 2019·Proceedings of the National Academy of Sciences of the United States of America·Xiaobo ZhaoJoanne Chory
Oct 21, 2019·The Plant Journal : for Cell and Molecular Biology·Ian D SmallOren Ostersetzer-Biran
Jul 25, 2019·Journal of Integrative Plant Biology·Hong-Chun WangBao-Cai Tan
Aug 30, 2017·The Plant Journal : for Cell and Molecular Biology·Chao HuangZhong-Nan Yang
Oct 17, 2017·The Plant Journal : for Cell and Molecular Biology·Michael F DiazR Michael Mulligan
Sep 25, 2019·International Journal of Molecular Sciences·Faan TianYing Miao
Apr 4, 2017·Wiley Interdisciplinary Reviews. RNA·Xiaowen ShiStephane Bentolila
Jul 20, 2017·Scientific Reports·Meijie LuoFazhan Qiu
Oct 28, 2017·Science China. Life Sciences·Junjie YanPing Yin
May 12, 2019·The Plant Journal : for Cell and Molecular Biology·Rafael SandovalMichael L Hayes
Oct 27, 2018·Nucleic Acids Research·Man LiZhang Zhang
Jun 21, 2020·International Journal of Molecular Sciences·Xiaowei ZhuGuoan Zhou
Dec 16, 2020·International Journal of Molecular Sciences·Yuzhe SunBoon Leong Lim
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Oct 31, 2016·Trends in Plant Science·Tao SunMaureen R Hanson
Jun 9, 2020·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Jingliang SunJian-Xiang Liu

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Methods Mentioned

BETA
transfections
transfection
transgenic
immunoprecipitation
GTPase
two-hybrid
RIP-RIP
Y2H
PCR

Software Mentioned

Blast
GeneDoc
MASCOT
MEME
Phylodendron
coffee
ImageQuant
Clustal Omega

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