PMID: 2491924Jan 1, 1989Paper

A "zinc-finger"-type binding domain in tobacco streak virus coat protein

Virology
P C SehnkeJ E Johnson

Abstract

Tobacco streak virus (TSV) RNA and alfalfa mosaic virus (AIMV) RNA will replicate only if a few copies of their coat proteins are bound to the RNA. To understand this phenomenon experiments were performed to find unique features of the TSV and AIMV subunits. Atomic absorption analysis showed that TSV and AIMV contained substantial quantities of zinc in native virions (approximately one zinc atom per four protein subunits in TSV and one zinc atom per two protein subunits in AIMV), while other plant viruses tested did not. Treatment of TSV with a zinc-extracting reagent resulted in partial degradation of all the TSV nucleoprotein components, although the top component was most effected. The sequence (Cys X2 Cys X10 Cys X2 His) was found between residues 28 and 45 in the TSV primary structure and it is similar to a sequence found in several nucleic acid-binding, gene-regulatory proteins, most notably transcription factor IIIA from Xenopus laevis. TSV subunits were found to be extensively crosslinked within the virions. TSV and AIMV contain sequences rich in basic residues in the amino-terminal portion of the subunit (residues 51 to 72 in TSV and 1 to 26 in AIMV) and helical predictions suggested modes of protein-nucleic acid inter...Continue Reading

References

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Citations

Jan 1, 1995·Archives of Virology·P J ShielP H Berger
Aug 1, 1992·Virus Genes·G D Foster, P R Mills
Jan 16, 2014·Indian Journal of Virology : an Official Organ of Indian Virological Society·Saurabh KulshresthaAijaz Asghar Zaidi
Nov 11, 1989·Nucleic Acids Research·L Wakefield, G G Brownlee
Dec 27, 2015·Journal of Structural Biology·Ashutosh GulatiM R N Murthy
Sep 1, 1990·Virology·U Niesbach-KlösgenK Richards

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