PMID: 9450817Feb 5, 1998Paper

A(1,2)BO(1,2) genotyping by multiplexed allele-specific PCR

British Journal of Haematology
S L Pearson, M J Hessner

Abstract

The ABO blood group is the most clinically important human alloantigen system in transfusion medicine. The system involves three antigens A, B and H. H antigen is converted to either A or B by the activity of alpha1-->3-N-acetyl-galactosaminyl transferase (A transferase) or alpha1-->3 galactosyl transferase (B transferase). The O phenotype is the result of an inactive glycosyltransferase, which is unable to glycosylate the H antigen. The immunological properties of the ABO system were identified at the turn of the century; however, the genetic basis of the ABO system has only recently been characterized. This has enabled the development of a number of molecular ABO typing methods. Described here is a two-reaction multiplex allele-specific PCR (ASPCR) genotyping assay for the A1, A2, B, O1 and O2 subtypes. 11 different allele-specific oligonucleotide primers were selected to detect the presence or absence of the O1 associated G--> (-) deletion at base 261, the O2 associated G --> A substitution at base 802, the B associated G --> A substitution at base 803, and finally the A2 associated C --> (-) deletion at base 1059. A total of 122 peripheral blood samples were genotyped and serologically forward and reverse typed. A concordan...Continue Reading

References

May 17, 1990·Nature·F YamamotoS Hakomori
Jan 1, 1995·Vox Sanguinis·F Yamamoto
Feb 1, 1995·Glycobiology·F YamamotoS Hakomori
Jan 1, 1993·Human Mutation·D S O'Keefe, A Dobrovic
Jan 1, 1996·Medical and Veterinary Entomology·D B TaylorR D Peterson
Jan 1, 1996·Vox Sanguinis·M L Olsson, M A Chester

❮ Previous
Next ❯

Citations

Feb 6, 2008·International Journal of Hematology·Mokhtar M El-Zawahri, Yunus A Luqmani
Jun 14, 2005·Legal Medicine·Koichi Suzuki
Mar 25, 2014·Legal Medicine·Takashi Taki, Kazuhiko Kibayashi
Aug 16, 2003·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·J Downing, C Darke
Nov 1, 2009·Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology·Theresa A Salerno
Mar 4, 2008·Haemophilia : the Official Journal of the World Federation of Hemophilia·A SharthkumarA Shapiro
Jan 14, 2011·Journal of Forensic Sciences·Hwan Young LeeKyoung-Jin Shin
May 29, 2004·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Nicolas von BeckerathAdnan Kastrati
Jul 30, 2009·Electrophoresis·James Chun-I LeeLi-Chin Tsai
Dec 17, 2009·Journal of Forensic Sciences·Li RuanQingge Li
Jul 13, 2005·Transfusion Medicine Reviews·Mark H Yazer
Aug 12, 2009·Molecular and Cellular Probes·Yiran WangY-H Percival Zhang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.