A20 inhibits the motility of HCC cells induced by TNF-α

Oncotarget
Xianteng WangYongyu Shi

Abstract

Metastasis of hepatocellular carcinoma (HCC) can be facilitated by TNF-α, a prototypical inflammatory cytokine in the HCC microenvironment. A20 is a negative regulator of NF-κB signaling pathway. In the present study we ask whether A20 plays a role in HCC metastasis. We found that A20 expression was downregulated in the invasive cells of microvascular invasions (MVI) compared with the noninvasive cells in 89 tissue samples from patients with HCC by immunochemistry methods. Overexpression of A20 in HCC cell lines inhibited their motility induced by TNF-α. Furthermore, the overexpression of A20 inhibited epithelial-mesenchymal transition (EMT), FAK activation and RAC1 activity. By contrast, knockdown of A20 in one HCC cell line results in the converse. In addition, the overexpression of A20 restrained the formation of MVI in HCC xenograft in nude mice treated with TNF-α. All the results suggested that A20 functioned as a negative regulator in motility of HCC cells induced by TNF-α.

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Citations

Jun 7, 2018·NPJ Precision Oncology·Le-Xing YuHong-Yang Wang
May 28, 2019·Journal of Cellular Physiology·Peng Sheng YiJian Shui Li
Nov 23, 2019·Journal of Receptor and Signal Transduction Research·Nguyen Thi XuanNguyen Huy Hoang
Apr 4, 2021·Biomedicines·Seung Wan SonJong Kook Park
May 3, 2021·Cancer Letters·Yongyu ShiLining Zhang

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Methods Mentioned

BETA
xenografts
transfection
deubiquitination
ubiquitination
GTPases
pull-down
xenograft
xenograf
PCR
electrophoresis

Software Mentioned

GraphPad Prism
GelPro
Pro Plus
Image

Related Concepts