PMID: 2115300Jul 1, 1990Paper

A23187 increases permeability of MDCK monolayers independent of phospholipase activation

The American Journal of Physiology
M W Peterson, D Gruenhaupt

Abstract

Changes in intracellular calcium influence epithelial barrier integrity, but the mechanism of action is unknown. One possibility is that calcium may work by increasing phospholipase A2 (PLA2) and/or phospholipase C (PLG) activity. Measuring the mannitol permeability (Pmann) of cultured monolayers of Madin-Darby canine kidney (MDCK) epithelium cells as a measure of barrier integrity, we found that exposure of the monolayers to 5 and 10 microM A23187 produced an increase in Pmann whereas 1 microM A23187 did not. Exposure of MDCK cells labeled with [3H]arachidonate to A23187 resulted in an increase in both PLA2 activity, as measured by an increase in free fatty acids, and in PLC activity, as measured by an increase in diacylglycerol (DAG). The increase in DAG was due to an increase in phosphatidylcholine-specific PLC activity. The relationship of phospholipolysis to Pmann was evaluated further by the use of mepacrine and dexamethasone. Mepacrine (10 microM) decreased PLA2 activity by 60% but had no effect on increased Pmann after exposure to A23187. Preexposure of the monolayers to dexamethasone (10 microM) blocked both PLA2 activity and PLC activity and also prevented the increase in Pmann after exposure to A23187. To evaluate wh...Continue Reading

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Citations

Nov 1, 1993·American Journal of Respiratory Cell and Molecular Biology·T RochatP Meda
Apr 28, 2012·Pflügers Archiv : European journal of physiology·Douglas K Atchison, William H Beierwaltes
Aug 7, 2013·Current Opinion in Nephrology and Hypertension·Pascal Houillier
Nov 20, 1998·The American Journal of the Medical Sciences·M A Baum, H W Harris

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