A2E selectively induces cox-2 in ARPE-19 and human neural cells

Current Eye Research
Walter J LukiwNicolas G Bazan

Abstract

To investigate the expression of cyclooxygenase (COX)-1, -2, and -3 RNA and protein in retinal pigment epithelial (ARPE-19) cells and in human neural (HN) cells exposed to the stress-inducing cytokines IL-1beta and TNF-a, the oxidizing peroxide H(2)O(2), the combination of TNF-alpha + H(2)O(2), and the lipofuscin fluorophore A2E. Three-week-old ARPE-19 and HN cells were incubated with IL-1beta (10 ng/ml), TNF-alpha (10 ng/ml), H(2)O(2) (0.6 microM), TNF-alpha + H(2)O(2) (10 ng/ml and 0.6 microM), or A2E (10 microM) for 8 hr, after which total RNA and whole cellular proteins were isolated. Cyclooxygenase-1, -2, and -3 RNA and protein levels were quantified using Northern and Western immunoassay. IL-1beta-, H(2)O(2)-, TNF-alpha-, TNF-alpha + H(2)O(2)-, or A2E-stressed ARPE-19 or HN cells displayed no significant upregulation in COX-1 or COX-3 RNA message abundance; however, significant upregulation was observed in COX-2 RNA message and protein abundance. A2E treatment of HN cells resulted in modest increases in COX-3 protein, an effect that was not observed in ARPE-19 cells. COX-2 RNA levels were induced in cytokine-, peroxide-, and A2E-stressed ARPE-19 and HN cells. Lack of induction of COX-3 RNA message by A2E, coupled with inc...Continue Reading

References

Aug 8, 1997·Biochemical and Biophysical Research Communications·R NewtonP J Barnes
Jan 28, 1999·Biochemical and Biophysical Research Communications·Y KitamuraT Taniguchi
Oct 12, 2000·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·M Katori, M Majima
Feb 8, 2003·Annual Review of Biophysics and Biomolecular Structure·R Michael Garavito, Anne M Mulichak
Sep 26, 2003·Investigative Ophthalmology & Visual Science·Walter J LukiwNicolas G Bazan
Nov 13, 2003·Vision Research·Janet R SparrowKoji Nakanishi
Jun 15, 2004·Neuroreport·Aileen I Pogue, Walter J Lukiw
Aug 20, 2004·Pharmacological Reviews·Daniel L SimmonsTimothy Hla
Sep 1, 2004·Experimental Eye Research·E R GaillardJ P Dillon
Sep 30, 2004·Neurochemical Research·Jian-Guo CuiWalter J Lukiw
Dec 8, 2004·Current Medicinal Chemistry·W Kiefer, G Dannhardt
May 3, 2005·Experimental Eye Research·Janet R Sparrow, Mike Boulton
May 4, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Wanzhong WangJan-Erik Damber

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Citations

Jul 25, 2014·Cell Death & Disease·D M Mihai, I Washington
Jul 28, 2015·Journal of Inorganic Biochemistry·A I PogueW J Lukiw
May 28, 2016·Journal of Tissue Engineering and Regenerative Medicine·Anat YanaiKevin Gregory-Evans

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