AAV-Mediated Astrocyte-Specific Gene Expression under Human ALDH1L1 Promoter in Mouse Thalamus

Experimental Neurobiology
Wuhyun KohC Justin Lee

Abstract

Adeno-associated virus (AAV)-mediated gene delivery has been proposed to be an essential tool of gene therapy for various brain diseases. Among several cell types in the brain, astrocyte has become a promising therapeutic target for brain diseases, as more and more contribution of astrocytes in pathophysiology has been revealed. Until now, genetically targeting astrocytes has been possible by utilizing the glial fibrillary acidic protein (GFAP) promoter. In some brain areas including thalamus, however, the GFAP expression in astrocytes is reported to be low, making it difficult to genetically target astrocytes using GFAP promoter. To study the function of astrocytes in thalamus, which serves as a relay station, there is a great need for identifying an alternative astrocyte-specific promoter in thalamus. Recently, a new astrocyte-specific promoter of ALDH1L1 has been identified. However, it has not been examined in thalamus. Here we developed and characterized an AAV vector expressing Cre recombinase under the human ALDH1L1 promoter, AAV-hALDH1L1-Cre. To test the cell-type specific expression of AAV-hALDH1L1-Cre, AAV virus was injected into several brain regions of Ai14 (RCL-tdTomato) mouse, which reports Cre activity by tdTomat...Continue Reading

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Citations

Mar 10, 2020·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Hemil GonzalezJennillee Wallace
Feb 12, 2020·Nature Reviews. Neuroscience·Xinzhu YuBaljit S Khakh
Dec 19, 2019·Frontiers in Neuroanatomy·Leila HaeryMelina Fan
Nov 27, 2020·Journal of Neuroscience Methods·Antonio LuchicchiNathan Marchant
Jan 29, 2021·Frontiers in Molecular Neuroscience·Simon J O'CarrollDeborah Young
Jul 3, 2021·Cells·Anastasia A BorodinovaOlga L Vlasova
Jul 10, 2021·Frontiers in Cellular Neuroscience·Georg von JonquieresGary D Housley
Aug 13, 2021·Frontiers in Cellular Neuroscience·Aaron J MoulsonPeggy Assinck

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Methods Mentioned

BETA
transgenic

Software Mentioned

- browser2
ImageJ
Vistabrowser2
Allen Brain Atlas ( Atlas
VISTA

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