Ab initio folding of a trefoil-fold motif reveals structural similarity with a β-propeller blade motif.

Protein Science : a Publication of the Protein Society
Connie A TenorioMichael Blaber

Abstract

Many protein architectures exhibit evidence of internal rotational symmetry postulated to be the result of gene duplication/fusion events involving a primordial polypeptide motif. A common feature of such structures is a domain-swapped arrangement at the interface of the N- and C-termini motifs and postulated to provide cooperative interactions that promote folding and stability. De novo designed symmetric protein architectures have demonstrated an ability to accommodate circular permutation of the N- and C-termini in the overall architecture; however, the folding requirement of the primordial motif is poorly understood, and tolerance to circular permutation is essentially unknown. The β-trefoil protein fold is a threefold-symmetric architecture where the repeating ~42-mer "trefoil-fold" motif assembles via a domain-swapped arrangement. The trefoil-fold structure in isolation exposes considerable hydrophobic area that is otherwise buried in the intact β-trefoil trimeric assembly. The trefoil-fold sequence is not predicted to adopt the trefoil-fold architecture in ab initio folding studies; rather, the predicted fold is closely related to a compact "blade" motif from the β-propeller architecture. Expression of a trefoil-fold seq...Continue Reading

References

Oct 9, 1979·Journal of Molecular Biology·A D McLachlan
Mar 14, 1972·Journal of Molecular Biology·A D McLachlan
Dec 1, 1995·Protein Science : a Publication of the Protein Society·M J BennettD Eisenberg
Feb 20, 1996·Biochemistry·M BlaberK A Thomas
Dec 23, 1999·Current Opinion in Structural Biology·V Fülöp, D T Jones
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·N L OgiharaD Eisenberg
Aug 22, 2001·Protein Science : a Publication of the Protein Society·J Jung, B Lee
Jan 22, 2002·FEBS Letters·Birte HöckerReinhard Sterner
May 17, 2002·Structure·Frederic RousseauLaura S Itzhaki
Aug 4, 2006·Nucleic Acids Research·Jinn-Moon Yang, Chi-Hua Tung
Oct 24, 2006·Journal of Molecular Biology·Itamar Yadid, Dan S Tawfik
Nov 6, 2007·Proteins·Indronil ChaudhuriAndrei N Lupas
Jun 28, 2008·Science·Alexey G Murzin
Aug 25, 2009·Proteins·Srivatsan RamanDavid Baker
Apr 7, 2010·Proceedings of the National Academy of Sciences of the United States of America·Itamar YadidDan S Tawfik
Aug 18, 2010·Protein Engineering, Design & Selection : PEDS·Itamar Yadid, Dan S Tawfik
Dec 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jihun Lee, Michael Blaber
Jul 14, 2012·Cellular and Molecular Life Sciences : CMLS·Michael BlaberLiam Longo
Sep 17, 2013·Structure·Yifan SongDavid Baker
Oct 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Arnout R D VoetJeremy R H Tame
Jun 21, 2015·Current Opinion in Structural Biology·Santhanam Balaji
Nov 27, 2015·Protein Science : a Publication of the Protein Society·Xue XiaMichael Blaber
Jan 7, 2016·Bioinformatics·Karolis Uziela, Björn Wallner
Jun 2, 2017·Biochemistry·Muralikrishna Lella, Radhakrishnan Mahalakshmi

❮ Previous
Next ❯

Citations

May 4, 2020·Protein Science : a Publication of the Protein Society·Connie A TenorioMichael Blaber
Jun 17, 2020·Protein Science : a Publication of the Protein Society·Michael Blaber
Oct 3, 2020·Protein Science : a Publication of the Protein Society·Bram MylemansArnout R D Voet
Mar 10, 2021·Protein Science : a Publication of the Protein Society·Michael Blaber
Mar 14, 2021·Proteins·Jeroen P M VranckenArnout R D Voet
Dec 30, 2020·Current Opinion in Structural Biology·Bram MylemansJeremy Rh Tame

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.