ABA Receptor Subfamily III Enhances Abscisic Acid Sensitivity and Improves the Drought Tolerance of Arabidopsis

International Journal of Molecular Sciences
Xiaoyi LiYi Yang

Abstract

The phytohormone abscisic acid (ABA) regulates plant growth, the developmental process, and abiotic stresses. ABA signaling is induced in response to mediate plant acclimation to environmental challenges, including high salinity and drought. The ABA-binding receptors (RCAR/PYR1/PYL), composing of 14 members, are the core components of the ABA-signaling pathway. Here, we observed that the three subfamilies within the RCARs showed different expression patterns at the basal and exogenous ABA levels. Subsequently, we generated transgenic plants overexpressing subfamily III, RCAR11⁻RCAR14, respectively. The transgenic plants showed increased ABA sensitivity in seed germination and post-germination seedling establishment and root length. Further studies revealed that the overexpressing subfamily III transgenic plants enhanced drought resistance, increased water-use efficiency, and accelerated stress-responsive gene expression compared with the wild-type plants. These findings confirm that the subfamily III plays a key role in ABA-mediated developmental processes and, more importantly, is involved in drought tolerance in the ABA-dependent pathway.

References

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Citations

Apr 11, 2019·Antioxidants·Miriam LaxaKarl-Josef Dietz
Jan 30, 2020·Journal of Experimental Botany·Kris VissenbergSébastjen Schoenaers
Sep 23, 2018·International Journal of Molecular Sciences·Iva PavlovićBranka Salopek-Sondi
Dec 19, 2019·Frontiers in Plant Science·Rakesh Kumar VermaViswanathan Chinnusamy
Jul 6, 2021·Plant Signaling & Behavior·Qin YuXiaoyi Li

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Methods Mentioned

BETA
transfection
ubiquitination
transgenic
PCR

Software Mentioned

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