ABC multidrug transporter Cdr1p of Candida albicans has divergent nucleotide-binding domains which display functional asymmetry

FEMS Yeast Research
Sudhakar JhaRajendra Prasad

Abstract

In order to ascertain the molecular basis of ATP-mediated drug extrusion by Cdr1p, a multidrug transporter of Candida albicans, we recently have reported that the Walker A motif of the N-terminal nucleotide biding domain (NBD) of this protein contains an uncommon cysteine residue (C193; GXXGXGCS/T) which is indispensable for ATP hydrolysis. This residue is exceptionally conserved in N-terminal NBDs of fungal ABC transporters and hence makes these transporters an evolutionarily divergent group. However, the presence of a conventional lysine residue at a similar position in the Walker A motif of the C-terminal NBD warrants the individual contribution of both the NBDs in the ATP-driven efflux function of such transporters. In this study we have investigated the contribution of this divergent Walker A motif in the context of the full Cdr1p protein under in vivo conditions by swapping these two crucial amino acids (C193K in Walker A motif of N-terminal NBD and K901C in Walker A motif of C-terminal NBD) between the two NBDs. Both the native and the mutant variants of Cdr1p were integrated at the PDR5 locus as GFP-tagged fusion proteins and were hyper-expressed. Our study shows that both C193K- and K901C-expressing cells elicit a seve...Continue Reading

References

Jan 1, 1988·Methods in Enzymology·A Goffeau, J P Dufour
Apr 1, 1987·Journal of Clinical Microbiology·S Scherer, D A Stevens
Mar 1, 1995·Antimicrobial Agents and Chemotherapy·K V Clemons, D A Stevens
Feb 1, 1997·Nature Genetics·A Decottignies, A Goffeau
Jun 20, 1998·Clinical Microbiology Reviews·T C WhiteR A Bowden
Jun 21, 2002·Advances in Microbial Physiology·Rajendra Prasad Smriti
Nov 19, 2002·Antimicrobial Agents and Chemotherapy·Kasturi MukhopadhyayRajendra Prasad
Oct 11, 2003·Biochemical and Biophysical Research Communications·Sudhakar JhaRajendra Prasad
Dec 11, 2003·Eukaryotic Cell·Suneet ShuklaRajendra Prasad

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Citations

Mar 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Robert ErnstLutz Schmitt
Feb 19, 2009·Antimicrobial Agents and Chemotherapy·Sarah TsaoMartine Raymond
Jun 19, 2012·Annual Review of Microbiology·Rajendra Prasad, Andre Goffeau
Nov 7, 2006·Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology·Ann R HolmesRichard D Cannon
Sep 16, 2014·Frontiers in Pharmacology·Rajendra Prasad, Manpreet K Rawal
Sep 27, 2015·Eukaryotic Cell·Rajendra PrasadSanjiveeni Dhamgaye
May 4, 2005·Yeast
Sep 8, 2018·Yeast·Rajendra PrasadNitesh Kumar Khandelwal
Jan 29, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Erik ProckoRachelle Gaudet
Aug 30, 2008·Bioscience Reports·Sneh Lata PanwarRajendra Prasad

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