ABC Transporter Genes Show Upregulated Expression in Drug-Resistant Clinical Isolates of Candida auris : A Genome-Wide Characterization of ATP-Binding Cassette (ABC) Transporter Genes

Frontiers in Microbiology
Mohd WasiRajendra Prasad

Abstract

ATP-binding cassette (ABC) superfamily members have a key role as nutrient importers and exporters in bacteria. However, their role as drug exporters in eukaryotes brought this superfamily member to even greater prominence. The capacity of ABC transporters to efflux a broad spectrum of xenobiotics represents one of the major mechanisms of clinical multidrug resistance in pathogenic fungi including Candida species. Candida auris, a newly emerged multidrug-resistant fungal pathogen of humans, has been responsible for multiple outbreaks of drug-resistant infections in hospitals around the globe. Our study has analyzed the entire complement of ABC superfamily transporters to assess whether these play a major role in drug resistance mechanisms of C. auris. Our bioinformatics analyses identified 28 putative ABC proteins encoded in the genome of the C. auris type-strain CBS 10913T; 20 of which contain transmembrane domains (TMDs). Quantitative real-time PCR confirmed the expression of all 20 TMD transporters, underlining their potential in contributing to the C. auris drug-resistant phenotype. Changes in transcript levels after short-term exposure of drugs and in drug-resistant C. auris isolates suggested their importance in the drug ...Continue Reading

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Citations

Aug 7, 2020·FEMS Yeast Research·Garima ShahiRajendra Prasad
Apr 3, 2020·Expert Review of Anti-infective Therapy·Arunaloke Chakrabarti, Shreya Singh
Aug 19, 2020·Journal of Bioenergetics and Biomembranes·Kseniia V GalkinaDmitry A Knorre
May 1, 2020·Frontiers in Genetics·Aleksandra D ChybowskaRhys A Farrer
Dec 18, 2020·PLoS Pathogens·Zoe K Ross, Alexander Lorenz
Feb 19, 2021·Journal of Medical Microbiology·Arunaloke Chakrabarti, Prashant Sood
Mar 7, 2021·Fungal Genetics and Biology : FG & B·Sonam KumariRajendra Prasad
Feb 24, 2021·Antimicrobial Agents and Chemotherapy·Cheryl LeongThomas L Dawson
May 1, 2021·International Journal of Molecular Sciences·Lucia ČernákováCélia F Rodrigues
May 4, 2021·Frontiers in Cellular and Infection Microbiology·Sabrina JenullKarl Kuchler
May 7, 2021·Biochimica Et Biophysica Acta. Biomembranes·Poonam VishwakarmaAtanu Banerjee

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

BLASTp
TMPRED
GraphPad prism
Velvet
ClustalΩ
FASTX
Cytoscape
WoLF PSORT
MEGA6
MEGA

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