ABC transporters do not contribute to extracellular translocation of hyaluronan in human breast cancer in vitro

Experimental Cell Research
Natalie K Thomas, Tracey J Brown


Extracellular translocation of the polysaccharide, hyaluronan (HA) has been thought to be mediated via its transmembrane synthetic enzyme, hyaluronan synthase (HAS) but recent studies have indicated that the ATP-Binding-Cassette (ABC) transporter, MRP5 contributes to this process. Liberated and cell-associated HA contributes to breast cancer initiation and progression, and therefore the inhibition of ABC transporters and consequently HA transport could provide therapeutic benefit in the treatment of breast cancer. Quantitation of ABC transporter genes, MRP1-5, BCRP and MDR1 were determined in six breast cancer cell lines selected for their differential HA synthetic rates. Low endogenous expression of transporters was detected but no significant correlation existed between ABC transporter and HAS gene expression or HA production. A dose titration of up to ten times the IC(50) of ten small molecule ABC transporter inhibitors did not significantly inhibit HA export in four breast cancer cell lines. Unlike the changes observed after inhibition of HA synthesis by the characterised inhibitor 4-MU, inhibition of ABC transporters did not alter the cell morphology, HA glycocalyx or the intracellular quantity or localisation of HA. Colle...Continue Reading


Dec 2, 1992·International Journal of Cancer. Journal International Du Cancer·J PontingS Kumar
May 1, 1992·The Journal of Experimental Medicine·B A Dougherty, I van de Rijn
Jan 1, 1993·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·B DelpechP Creissard
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·W KnudsonC B Knudson
Sep 25, 1998·The Journal of Biological Chemistry·V L Tlapak-SimmonsP H Weigel
Aug 24, 1999·The Journal of Biological Chemistry·N ItanoK Kimata
Mar 30, 2001·The Journal of Biological Chemistry·V B LokeshwarB L Lokeshwar
Jan 23, 2003·The Journal of Pharmacology and Experimental Therapeutics·Maciej J Zamek-GliszczynskiKim L R Brouwer
Mar 26, 2003·The Journal of Biological Chemistry·Philip E Pummill, Paul L DeAngelis
May 27, 2003·The Journal of Biological Chemistry·Jason S PatzlaffBert Poolman
Dec 4, 2003·The International Journal of Biochemistry & Cell Biology·Jing BaiTheresa M C Tan
Jun 25, 2004·Glycobiology·Galina OuskovaPeter Prehm
Jul 9, 2004·Biochimica Et Biophysica Acta·Vincent C HascallThomas N Wight
Jul 8, 2005·Applied and Environmental Microbiology·Bill WidnerSteve Brown
Aug 30, 2005·Experimental Cell Research·Lishanthi UdabageTracey J Brown
Feb 23, 2007·International Journal of Cancer. Journal International Du Cancer·Yuejuan LiParaskevi Heldin
Jun 2, 2007·The Journal of Biological Chemistry·Tobias SchulzPeter Prehm
Jun 21, 2007·Molecular Microbiology·Roger J P DawsonKaspar P Locher
Nov 6, 2007·The Journal of Biological Chemistry·Paul H Weigel, Paul L DeAngelis
Jan 22, 2008·Arthritis Research & Therapy·Barthold Deiters, Peter Prehm
Jul 24, 2008·Cellular Signalling·Julie G DonaldsonLee Ann Cohen
Sep 20, 2008·Journal of Cellular Biochemistry·Katharina MonzPeter Prehm
Oct 7, 2008·Biochimica Et Biophysica Acta·E A DisalvoG J Gordillo
Aug 20, 2009·Mini Reviews in Medicinal Chemistry·L GattiP Perego

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