ATP-binding-cassette transporter 1 (ABC1) has been implicated in processes related to membrane-lipid turnover. Here, using in vivo loss-of-function and in vitro gain-of-function models, we show that ABC1 promotes Ca2+-induced exposure of phosphatidylserine at the membrane, as determined by a prothrombinase assay, membrane microvesiculation and measurement of transbilayer redistribution of spin-labelled phospholipids. That ABC1 promotes engulfment of dead cells is shown by the impaired ability of ABC1-deficient macrophages to engulf apoptotic preys and by the acquisition of phagocytic behaviour by ABC1 transfectants. Release of membrane phospholipids and cholesterol to apo-AI, the protein core of the cholesterol-shuttling high-density lipoprotein (HDL) particle, is also ABC1-dependent. We propose that both the efficiency of apoptotic-cell engulfment and the efflux of cellular lipids depend on ABC1-induced perturbation of membrane phosphatidylserine turnover. Transient local exposure of anionic phospholipids in the outer membrane leaflet may be sufficient to alter the general properties of the membrane and thus influence discrete physiological functions.
Lysosome recruitment and fusion are early events required for trypanosome invasion of mammalian cells
ATP-dependent asymmetric distribution of spin-labeled phospholipids in the erythrocyte membrane: relation to shape changes
A new embryonic stem cell line from DBA/1lacJ mice allows genetic modification in a murine model of human inflammation
Giantin, a novel conserved Golgi membrane protein containing a cytoplasmic domain of at least 350 kDa
Comprehensive, serologically equivalent DNA typing for HLA-B by PCR using sequence-specific primers (PCR-SSP)
Targeted disruption of the murine erythroid band 3 gene results in spherocytosis and severe haemolytic anaemia despite a normal membrane skeleton
MDR1 P-glycoprotein is a lipid translocase of broad specificity, while MDR3 P-glycoprotein specifically translocates phosphatidylcholine
ABC1, an ATP binding cassette transporter required for phagocytosis of apoptotic cells, generates a regulated anion flux after expression in Xenopus laevis oocytes.
Defects in sensory and autonomic ganglia and absence of locus coeruleus in mice deficient for the homeobox gene Phox2a
Calcium induces phospholipid redistribution and microvesicle release in human erythrocyte membranes by independent pathways
Molecular cloning of the human ATP-binding cassette transporter 1 (hABC1): evidence for sterol-dependent regulation in macrophages
Insights into the function of Rim protein in photoreceptors and etiology of Stargardt's disease from the phenotype in abcr knockout mice
Patterns of programmed cell death in populations of developing spinal motoneurons in chicken, mouse, and rat
The Tangier disease gene product ABC1 controls the cellular apolipoprotein-mediated lipid removal pathway
Human ATP-binding cassette transporter 1 (ABC1): genomic organization and identification of the genetic defect in the original Tangier disease kindred
Engulfment of apoptotic cells involves the redistribution of membrane phosphatidylserine on phagocyte and prey
Transport of lipids from golgi to plasma membrane is defective in tangier disease patients and Abc1-deficient mice
High density lipoprotein deficiency and foam cell accumulation in mice with targeted disruption of ATP-binding cassette transporter-1
The role of vesicular transport in ABCA1-dependent lipid efflux and its connection with NPC pathways
P2X7 receptors regulate multiple types of membrane trafficking responses and non-classical secretion pathways
Phosphatidylserine exposure during apoptosis reflects bidirectional trafficking between plasma membrane and cytoplasm
ATP-binding cassette transporter A1 mediates the beneficial effects of the liver X receptor agonist GW3965 on object recognition memory and amyloid burden in amyloid precursor protein/presenilin 1 mice.
The yeast plasma membrane ATP binding cassette (ABC) transporter Aus1: purification, characterization, and the effect of lipids on its activity.
Interleukin-6 protects human macrophages from cellular cholesterol accumulation and attenuates the proinflammatory response.
Lipofuscin and N-retinylidene-N-retinylethanolamine (A2E) accumulate in retinal pigment epithelium in absence of light exposure: their origin is 11-cis-retinal.
Formation of two intramolecular disulfide bonds is necessary for ApoA-I-dependent cholesterol efflux mediated by ABCA1
Two alternative mechanisms that regulate the presentation of apoptotic cell engulfment signal in Caenorhabditis elegans
Cellular cholesterol substrate pools for adenosine-triphosphate cassette transporter A1-dependent high-density lipoprotein formation
Genome-wide identification of TAL1's functional targets: insights into its mechanisms of action in primary erythroid cells
Promoter polymorphisms in the ATP binding cassette transporter gene influence production of cell-derived microparticles and are highly associated with susceptibility to severe malaria in humans
Expression of reverse cholesterol transport proteins ATP-binding cassette A1 (ABCA1) and scavenger receptor BI (SR-BI) in the retina and retinal pigment epithelium
mRNA expression of genes involved in lipid efflux and matrix degradation in occlusive and ectatic atherosclerotic disease
Variations on a gene: rare and common variants in ABCA1 and their impact on HDL cholesterol levels and atherosclerosis
Vascular imaging of solid tumors in rats with a radioactive arsenic-labeled antibody that binds exposed phosphatidylserine
A novel compound inhibits reconstituted high-density lipoprotein assembly and blocks nascent high-density lipoprotein biogenesis downstream of apolipoprotein AI binding to ATP-binding cassette transporter A1-expressing cells
Pathogen destruction versus intracellular survival: the role of lipids as phagosomal fate determinants
The ABC transporter gene family of Caenorhabditis elegans has implications for the evolutionary dynamics of multidrug resistance in eukaryotes
ATP hydrolysis-dependent conformational changes in the extracellular domain of ABCA1 are associated with apoA-I binding.
Nascent high density lipoproteins formed by ABCA1 resemble lipid rafts and are structurally organized by three apoA-I monomers.
Effects of deletion of macrophage ABCA7 on lipid metabolism and the development of atherosclerosis in the presence and absence of ABCA1
Characterization of a cDNA encoding guinea pig I3 associated with the delayed-type hypersensitivity reaction
The multidrug transporter, P-glycoprotein, actively mediates cholesterol redistribution in the cell membrane
Essential role for TrpC5-containing extracellular vesicles in breast cancer with chemotherapeutic resistance
Comparison between Ca2+-induced scrambling of various fluorescently labelled lipid analogues in red blood cells
ATP-binding cassette transporter A7 enhances phagocytosis of apoptotic cells and associated ERK signaling in macrophages
Phosphatidylserine (PS) induces PS receptor-mediated macropinocytosis and promotes clearance of apoptotic cells
Apoptotic cleavage of cytoplasmic dynein intermediate chain and p150(Glued) stops dynein-dependent membrane motility
Release of lysophospholipid 'find-me' signals during apoptosis requires the ATP-binding cassette transporter A1
Unexpected requirement for a binding partner of the syntaxin family in phagocytosis by murine testicular Sertoli cells
Phosphatidylserine is a global immunosuppressive signal in efferocytosis, infectious disease, and cancer
The overexpression of an intracellular ABCA-like transporter alters phospholipid trafficking in Leishmania
A new role for BMP5 during limb development acting through the synergic activation of Smad and MAPK pathways
Cyclic GMP modulates store-operated calcium entry inducing phosphatidylserine translocation at the surface of megakaryocytic cells
Phosphatidylethanolamine targeting for cell death imaging in early treatment response evaluation and disease diagnosis
Conformational change of apolipoprotein A-I and HDL formation from model membranes under intracellular acidic conditions.
ABCA1-mediated cholesterol efflux generates microparticles in addition to HDL through processes governed by membrane rigidity.
The predominance of one of the SR-BI isoforms is associated with increased esterified cholesterol levels not apoptosis in mink testis.
Janus kinase 2 modulates the lipid-removing but not protein-stabilizing interactions of amphipathic helices with ABCA1.
Direct detection of ABCA1-dependent HDL formation based on lipidation-induced hydrophobicity change in apoA-I.
Identification and functional analysis of a naturally occurring E89K mutation in the ABCA1 gene of the WHAM chicken.
ABCA1-dependent lipid efflux to apolipoprotein A-I mediates HDL particle formation and decreases VLDL secretion from murine hepatocytes.
Synthetic amphipathic helical peptides promote lipid efflux from cells by an ABCA1-dependent and an ABCA1-independent pathway.
Selection for female traits of high fertility affects male reproductive performance and alters the testicular transcriptional profile
Preferential ATP-binding cassette transporter A1-mediated cholesterol efflux from late endosomes/lysosomes
Phosphatidylcholine transfer protein promotes apolipoprotein A-I-mediated lipid efflux in Chinese hamster ovary cells
Natural phosphatidylcholine is actively translocated across the plasma membrane to the surface of mammalian cells.
The trigger to cell death determines the efficiency with which dying cells are cleared by neighbours
IKCa1 activity is required for cell shrinkage, phosphatidylserine translocation and death in T lymphocyte apoptosis
Regulation of cholesterol homeostasis in macrophages and consequences for atherosclerotic lesion development
ATP-binding cassette transporter A1 is significantly involved in the intestinal absorption of alpha- and gamma-tocopherol but not in that of retinyl palmitate in mice
Localization of human ATP-binding cassette transporter 1 (ABC1) in normal and atherosclerotic tissues
High density lipoproteins and arteriosclerosis. Role of cholesterol efflux and reverse cholesterol transport
Induction of transglutaminase 2 by a liver X receptor/retinoic acid receptor alpha pathway increases the clearance of apoptotic cells by human macrophages
Glucocorticoid receptor regulates ATP-binding cassette transporter-A1 expression and apolipoprotein-mediated cholesterol efflux from macrophages
Small, dense high-density lipoprotein-3 particles are enriched in negatively charged phospholipids: relevance to cellular cholesterol efflux, antioxidative, antithrombotic, anti-inflammatory, and antiapoptotic functionalities
Macrophage ATP-binding cassette transporter A1 overexpression inhibits atherosclerotic lesion progression in low-density lipoprotein receptor knockout mice
Characterization of an ABCA-like transporter involved in vesicular trafficking in the protozoan parasite Trypanosoma cruzi
ABCA1 mediates unfolding of apolipoprotein AI N terminus on the cell surface before lipidation and release of nascent high-density lipoprotein
Necrotic Cells Actively Attract Phagocytes through the Collaborative Action of Two Distinct PS-Exposure Mechanisms
ATP-binding cassette transporter hallmarks tissue macrophages and modulates cytokine-triggered polarization programs
Annexin A5 down-regulates surface expression of tissue factor: a novel mechanism of regulating the membrane receptor repertoir
N-terminal mutation of apoA-I and interaction with ABCA1 reveal mechanisms of nascent HDL biogenesis.
ATP-binding cassette transporter 1 and transglutaminase 2 act on the same genetic pathway in the apoptotic cell clearance
If phosphatidylserine is the death knell, a new phosphatidylserine-specific receptor is the bellringer
ATP-binding cassette transporter A1: a cell cholesterol exporter that protects against cardiovascular disease
Lack of ABCA1 considerably decreases brain ApoE level and increases amyloid deposition in APP23 mice
ABCA1 mediates high-affinity uptake of 25-hydroxycholesterol by membrane vesicles and rapid efflux of oxysterol by intact cells
Transition from dimers to higher oligomeric forms occurs during the ATPase cycle of the ABCA1 transporter
ATP-binding cassette transporter A1 expression disrupts raft membrane microdomains through its ATPase-related functions
Functional implications of the influence of ABCA1 on lipid microenvironment at the plasma membrane: a biophysical study
Naturally occurring mutations in the largest extracellular loops of ABCA1 can disrupt its direct interaction with apolipoprotein A-I.
Inhibitors of ATP-binding cassette transporters suppress interleukin-12 p40 production and major histocompatibility complex II up-regulation in macrophages
Molecular and functional interaction of the ATP-binding cassette transporter A1 with Fas-associated death domain protein.
Protein kinase A site-specific phosphorylation regulates ATP-binding cassette A1 (ABCA1)-mediated phospholipid efflux.
Defective fetal liver erythropoiesis and T lymphopoiesis in mice lacking the phosphatidylserine receptor.
A novel missense mutation in ABCA1 results in altered protein trafficking and reduced phosphatidylserine translocation in a patient with Scott syndrome.
Transmission of Cricket paralysis virus via exosome-like vesicles during infection of Drosophila cells
Interaction of CED-6/GULP, an adapter protein involved in engulfment of apoptotic cells with CED-1 and CD91/low density lipoprotein receptor-related protein (LRP)
Loss of plasma membrane phospholipid asymmetry requires raft integrity. Role of transient receptor potential channels and ERK pathway
Regulation of phosphatidylserine transbilayer redistribution by store-operated Ca2+ entry: role of actin cytoskeleton
Endocytosis is enhanced in Tangier fibroblasts: possible role of ATP-binding cassette protein A1 in endosomal vesicular transport
Evidence for a central apolipoprotein A-I domain loosely bound to lipids in discoidal lipoproteins that is capable of penetrating the bilayer of phospholipid vesicles
Identification of LBM180, a lamellar body limiting membrane protein of alveolar type II cells, as the ABC transporter protein ABCA3.
The lipidation by hepatocytes of human apolipoprotein A-I occurs by both ABCA1-dependent and -independent pathways.
Unconventional protein secretion: membrane translocation of FGF-2 does not require protein unfolding
Functionally defective high-density lipoprotein: a new therapeutic target at the crossroads of dyslipidemia, inflammation, and atherosclerosis
Persistence of procoagulant surface expression on activated human platelets: involvement of apoptosis and aminophospholipid translocase activity
Identification of lipid-phosphatidylserine (PS) as the target of unbiasedly selected cancer specific peptide-peptoid hybrid PPS1
ATP binding cassette transporter ABCA1 modulates the secretion of apolipoprotein E from human monocyte-derived macrophages
Engulfment Genes Promote Neuronal Regeneration in Caenorhabditis Elegans: Two Divergent But Complementary Views
Evidence for a role of the adenosine 5'-triphosphate-binding cassette transporter A1 in the externalization of annexin I from pituitary folliculo-stellate cells
Appetizing rancidity of apoptotic cells for macrophages: oxidation, externalization, and recognition of phosphatidylserine
ATP-binding cassette transporter A1 contains an NH2-terminal signal anchor sequence that translocates the protein's first hydrophilic domain to the exoplasmic space
ABCA1 is essential for efficient basolateral cholesterol efflux during the absorption of dietary cholesterol in chickens.
ATP-binding cassette transporter A7 (ABCA7) binds apolipoprotein A-I and mediates cellular phospholipid but not cholesterol efflux.
The lipoprotein receptor-related protein-1 (LRP) adapter protein GULP mediates trafficking of the LRP ligand prosaposin, leading to sphingolipid and free cholesterol accumulation in late endosomes and impaired efflux
Sequential shrinkage and swelling underlie P2X7-stimulated lymphocyte phosphatidylserine exposure and death
Expression, localization, and functional model of cholesterol transporters in lactating and nonlactating mammary tissues of murine, bovine, and human origin
Efferocytosis of dying cells differentially modulate immunological outcomes in tumor microenvironment
Effects of mutations of ABCA1 in the first extracellular domain on subcellular trafficking and ATP binding/hydrolysis.
Clearance of apoptotic cells is not impaired in mouse embryos deficient in class A scavenger receptor types I and II (CD204)
Deficiency in the Lipid Exporter ABCA1 Impairs Retrograde Sterol Movement and Disrupts Sterol Sensing at the Endoplasmic Reticulum.
Low high-density lipoprotein cholesterol: physiological background, clinical importance and drug treatment
Formation of cholesterol-enriched structures by aberrant intracellular accumulation of ATP-binding cassette transporter A1
Enhanced apoA-I-dependent cholesterol efflux by ABCA1 from sphingomyelin-deficient Chinese hamster ovary cells
Phosphatidylserine exposure mediated by ABC transporter activates the integrin signaling pathway promoting axon regeneration
Engulfing cells promote neuronal regeneration and remove neuronal debris through distinct biochemical functions of CED-1
Resistance to UV-induced apoptosis in Chinese-hamster ovary cells overexpressing phosphatidylserine synthases
Secretory vesicular transport from the Golgi is altered during ATP-binding cassette protein A1 (ABCA1)-mediated cholesterol efflux.
ATP-binding cassette transporter 1 (ABCA1) deficiency decreases platelet reactivity and reduces thromboxane A2 production independently of hematopoietic ABCA1
The Ins and Outs of Cerebral Malaria Pathogenesis: Immunopathology, Extracellular Vesicles, Immunometabolism, and Trained Immunity
Macrophages and dendritic cells use different Axl/Mertk/Tyro3 receptors in clearance of apoptotic cells
Apoptotic cells trigger the ABCA1/STAT6 pathway leading to PPAR-γ expression and activation in macrophages
Structure-function relationships of apolipoprotein A-I: a flexible protein with dynamic lipid associations
Radiation-enhanced vascular targeting of human lung cancers in mice with a monoclonal antibody that binds anionic phospholipids
Antitumor effects of a monoclonal antibody that binds anionic phospholipids on the surface of tumor blood vessels in mice
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