Abstract
S1-M1-80 cells, derived from human colon carcinoma S1 cells, are mitoxantrone-selected ABCG2-overexpressing cells and are widely used in in vitro studies of multidrug resistance(MDR). In this study, S1-M1-80 cell xenografts were established to investigate whether the MDR phenotype and cell biological properties were maintained in vivo. Our results showed that the proliferation, cell cycle, and ABCG2 expression level in S1-M1-80 cells were similar to those in cells isolated from S1-M1-80 cell xenografts (named xS1-M1-80 cells). Consistently, xS1-M1-80 cells exhibited high levels of resistance to ABCG2 substrates such as mitoxantrone and topotecan, but remained sensitive to the non-ABCG2 substrate cisplatin. Furthermore, the specific ABCG2 inhibitor Ko143 potently sensitized xS1-M1-80 cells to mitoxantrone and topotecan. These results suggest that S1-M1-80 cell xenografts in nude mice retain their original cytological characteristics at 9 weeks. Thus, this model could serve as a good system for further investigation of ABCG2-mediated MDR.
References
Sep 1, 1994·Somatic Cell and Molecular Genetics·T SumizawaS Akiyama
Feb 13, 2001·Biochemical and Biophysical Research Communications·S KawabataS Kohno
Nov 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Johan W JonkerAlfred H Schinkel
Jun 24, 2003·The American Journal of Pathology·Koichi ShimanoTohru Tsujimura
Dec 11, 2003·Cancer Chemotherapy and Pharmacology·Liwu FuQichao Pan
Mar 27, 2004·The Journal of Biological Chemistry·Partha KrishnamurthyJohn D Schuetz
Dec 2, 2005·Cancer Biology & Therapy·Zhi ShiLi-wu Fu
Mar 7, 2006·Nature Reviews. Drug Discovery·Gergely SzakácsMichael M Gottesman
Oct 4, 2006·Physiological Reviews·Balázs SarkadiAndrás Váradi
Feb 3, 2007·Cancer Chemotherapy and Pharmacology·Chun-Ling DaiLi-Wu Fu
Aug 3, 2007·Oncology·Shigeru TsunodaYutaka Shimada
Sep 6, 2007·Cancer Biology & Therapy·Jian-ye ZhangLi-wu Fu
Oct 3, 2008·Cancer Research·Chun-ling DaiLi-wu Fu
Nov 28, 2008·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Young Hak KimAtsushi Ochiai
Jul 29, 2009·Hematology·Arcadi I SvirnovskiUladzimir U Fedasenka
Feb 4, 2010·Leukemia Research·Daniela DamianiRenato Fanin
Sep 30, 2010·Cancer Research·Yan-Jun MiLi-Wu Fu
Nov 22, 2011·Chinese Journal of Cancer·Takeo Nakanishi, Douglas D Ross