Aberrant activation of Notch-1 signaling inhibits podocyte restoration after islet transplantation in a rat model of diabetic nephropathy

Cell Death & Disease
Yunqiang HeTao Yang

Abstract

Signaling abnormalities play important roles during podocyte injury and have been indicated as crucial events for triggering many glomerular diseases. There is emerging evidence demonstrating significant improvements in preventing renal injury and restoring podocytes after islet transplantation. However, whether signaling abnormalities affect the therapeutic efficacy of islet transplantation remain unclear. This study was established to investigate the impact of Notch-1 signaling activation on renal injury and podocyte restoration after islet transplantation. Experiments were performed in vivo and in vitro under conditions of diabetic nephropathy and high-glucose medium, respectively. Podocyte injury in vitro was induced by high-glucose concentration, and expression levels of genes associated with the Notch-1 pathway were also regulated by Jagged-1/FC and N-[N-(3,5-Difluorophenacetyl)-L-alanyl]- S-phenylglycine t-butyl ester (DAPT). Podocytes were co-cultured with islets to investigate the protective effect of islets in high-glucose conditions. Histopathological staining and transmission electron microscopy were performed to assess pathological changes in podocytes in glomeruli. The results from this study showed that Notch-1 s...Continue Reading

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Citations

Jan 25, 2020·Molecular Medicine Reports·Xiaokai Duan, Guijun Qin
Jul 2, 2020·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Caigu HeYanfang Zheng
Feb 2, 2021·Frontiers in Pharmacology·Carlamaria ZojaDaniela Macconi

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Methods Mentioned

BETA
PCR
transmission electron microscopy
Assay
electrophoresis

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Pro Plus
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