Aberrant CCR4 expression is involved in tumor invasion of lymph node-negative human gastric cancer

PloS One
Yongmei YangChuanxin Wang

Abstract

Cellular chemotaxis is the best-known function of chemokine receptors which are closely linked with tumor metastasis. In fact, positive expression of chemokine receptors could also be identified even in some patients without metastatic tumors, while the clinical relevance of this data has not been fully established. Our studies were designed to clarify the CCR4 expression profiles and to explore its potential role in histologically node-negative (pN0) gastric cancer (GC). Immunohistochemistry (IHC) or immunohistofluorescence (IHF) analysis was performed on specimens obtained from 108 patients with pN0 GC. We found that CCR4 was aberrantly over-expressed inpN0 GC tissues, with different expression patterns on tumor cells and being associated with T-stage (P = 0.002). The matrigel in vitro invasion assay revealed that over-expression of CCR4 in GC cell lines significantly enhanced the invasive capacity of these cells. Results from real-time RT-PCR and gelatinzymography showed a significant increase in matrix metalloproteinase (MMP)-9 production induced by the forced expression of CCR4 in GC cell lines. Our data suggest that the aberrant CCR4 expression is involved in tumor invasion of pN0 GC and, conceivably, antagonists of CCR4 ...Continue Reading

References

Apr 27, 2000·The Journal of Biological Chemistry·W WangA Zlotnik
Apr 17, 2002·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Jin-Pok KimHan-Kwang Yang
Jun 22, 2002·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Ida R K BukholmJahn M Nesland
Jul 5, 2007·Journal of Gastroenterology and Hepatology·Chin-Chun LeeWing-Yiu Lui
Jan 29, 2008·International Journal of Cancer. Journal International Du Cancer·Yoshiki MizukamiHideki Fujii
Mar 25, 2009·Cancer Microenvironment : Official Journal of the International Cancer Microenvironment Society·Frank M SpeetjensPeter J K Kuppen
Jun 11, 2009·International Journal of Oncology·Takaaki ArigamiTakashi Aikou
Jul 9, 2010·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Jiang LiGuobin Wang
Nov 4, 2010·Cancer Science·Takashi Ishida, Ryuzo Ueda
Dec 25, 2010·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Miao-zhen QiuRui-hua Xu
Dec 25, 2010·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Miao-zhen QiuRui-hua Xu
Nov 28, 2012·International Journal of Cancer. Journal International Du Cancer·Takahiro TsujikawaYutaka Kawakami
Mar 23, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Wei-ning WangTie-hui Hu

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Citations

Sep 7, 2016·International Journal of Molecular Sciences·David A KircherSheri L Holmen
Sep 20, 2018·Journal of Cellular Biochemistry·Hongda ZhaoBenkang Shi
Mar 1, 2018·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Zhonghua SongShouhua Chen
Mar 27, 2019·The Journal of Clinical Investigation·Anne MonetteRéjean Lapointe
Mar 20, 2018·Oncology Letters·Bożenna MytarMonika Baj-Krzyworzeka
Apr 2, 2020·Cancer Biotherapy & Radiopharmaceuticals·Jingjing HuCaihong Guan

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Methods Mentioned

BETA
transfection
flow cytometry
light microscopy

Software Mentioned

SPSS

Related Concepts