Aberrant Expression of miR-362 Promotes Lung Cancer Metastasis through Downregulation of Sema3A

Journal of Immunology Research
Dan LuoZi-Ling Wang

Abstract

miR-362 is a recently discovered member of the microRNA family, and it modulates a variety of physical activities and plays an important role in the occurrence and development of many tumors. However, the biological functions of hsa-miR-362-5p in non-small-cell lung carcinoma (NSCLC) are unknown. Transwell assay and colony formation were used to determine the migration, invasion, and proliferation of NSCLC cells in vitro. A subcutaneous tumor model in nude mice was established to detect NSCLC tumor growth in vivo. The direct binding of miR-362 to the 3'UTR of Semaphorin 3A (Sema3A) was confirmed by luciferase reporter assay. In this study, we found that the level of miR-362 was higher in NSCLC tissues than in adjacent normal tissues and that the level of miR-362 expression was also elevated in five NSCLC cell lines (A549, 95-D, H1299, H292, and H460) relative to a human normal lung epithelial cell line (BEAS2B). Furthermore, miR-362 promoted NSCLC cell invasion, migration, and colony formation in vitro and tumor formation in vivo. Next, we identified the miR-362 target gene Sema3A, which is significantly correlated with metastasis. Sema3A expression was increased in normal tissues relative to NSCLC tissues. This result is consi...Continue Reading

References

Nov 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·George Adrian CalinCarlo M Croce
Apr 19, 2005·Hematology/oncology Clinics of North America·Gary M Strauss
Jun 10, 2005·Nature·Kathryn A O'DonnellJoshua T Mendell
Oct 13, 2006·Cancer Chemotherapy and Pharmacology·Kaori Fujimoto-OuchiYutaka Tanaka
Mar 6, 2009·Nature Reviews. Cancer·Milena S NicolosoGeorge A Calin
Jul 28, 2009·Annual Review of Medicine·Ramiro GarzonCarlo M Croce
Jul 7, 2010·Molecular Cell·Yujing ZhangChen-Yu Zhang
Jul 22, 2010·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Ahmedin JemalElizabeth M Ward
Mar 24, 2011·The Cancer Journal·Armin GergerHeinz-Josef Lenz
Jan 3, 2013·International Journal of Cancer. Journal International Du Cancer·Lise Lotte ChristensenClaus L Andersen
Sep 24, 2013·Molecular Cell·Amanda N KallenYingqun Huang
Oct 26, 2013·Nature Protocols·F Ann RanFeng Zhang
Feb 1, 2014·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Andrew R Bassett, Ji-Long Liu
Apr 17, 2014·Cancer Cell·Weiying ZhouShizhen Emily Wang
Jul 23, 2014·RNA·Ashley L Cornett, Carol S Lutz
Jan 22, 2015·Immunity·Stephane PelletierDouglas R Green
Feb 6, 2015·Digestive Diseases and Sciences·Qing-Hui ZhangLing Xu
Mar 31, 2015·International Journal of Molecular Medicine·Haiyan JiangQinghua Xi
Jul 28, 2016·Journal of Cancer Research and Therapeutics·You-Kui ShiShi-Zhong Wang

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Citations

Apr 12, 2019·Frontiers in Cellular Neuroscience·Mengchen YangJianning Zhang
May 7, 2019·Molecular Medicine Reports·Yue LiXianghong Yang
Jan 2, 2019·International Journal of Molecular Sciences·Apoorva S Iyer, Svetlana P Chapoval
Jan 28, 2021·Cell Biology International·Neslisah BarlakOmer Faruk Karatas

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Methods Mentioned

BETA
Transfection
surgical resection
PCR
xenograft
reverse-transcription PCR

Software Mentioned

miRDB
miRanda
TargetScan
miRWalk

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