Aberrant expression of miR-663 and transforming growth factor-β1 in nasal polyposis in children

Experimental and Therapeutic Medicine
Hailing YuDa Li

Abstract

The aim of the present study was to investigate the expression of microRNA (miR)-663 and its regulatory effects on the pathogenesis of nasal polyposis in children. Nasal polyp tissue, as well as serum and peripheral blood eosinophils were collected from 35 children diagnosed with nasal polypectomy between August 2013 and August 2015. As a control, the inferior nasal concha, serum and peripheral blood eosinophils were collected from 46 patients with nasal septal deviation complicated by inferior turbinate hypertrophy or patients with simple inferior turbinate hypertrophy who had undergone surgical removal of the inferior nasal concha. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to measure the expression of miR-663 and transforming growth factor-β1 (TGF-β1) in the nasal polyp tissue, serum and peripheral blood eosinophils of patients with nasal polyposis and controls. Western blotting was used to measure the expression of TGF-β1 protein in nasal tissue and eosinophils and an enzyme-linked immunosorbent assay was used to measure serum level of TGF-β1 protein. A dual luciferase reporter assay was used to determine whether TGF-β1 was a target gene of miR-663. Compared with the control group, level...Continue Reading

References

Dec 1, 1995·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·J M BernsteinB Noble
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 9, 2003·The Annals of Otology, Rhinology, and Laryngology·Leif JohanssonMats Bende
Mar 12, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Iwona CichaChristoph D Garlichs
Oct 12, 2005·Current Allergy and Asthma Reports·Rafal PawliczakMarek L Kowalski
Apr 24, 2007·Current Allergy and Asthma Reports·Ruby Pawankar, Manabu Nonaka
Aug 20, 2009·Molecular and Cellular Biochemistry·Yan ShiKeke Huo
Aug 1, 2012·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Claus BachertPhilippe Gevaert
Feb 15, 2013·Genetics and Molecular Research : GMR·Z ZakariaB P Hoh
Dec 21, 2013·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Il-Ho ParkHeung-Man Lee
Aug 20, 2014·Nature Reviews. Rheumatology·Robert Lafyatis
Aug 26, 2014·The International Journal of Biochemistry & Cell Biology·Zhicheng XiaoJie Du
Dec 9, 2014·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K E HulseR P Schleimer
Jan 16, 2015·American Journal of Rhinology & Allergy·Jaewoon WeJeong-Whun Kim
Feb 24, 2015·Matrix Biology : Journal of the International Society for Matrix Biology·Sandrine Vadon-Le GoffCatherine Moali
Sep 8, 2015·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Sven BeckerMoritz Gröger
Sep 22, 2015·Frontiers in Medicine·Bram PiersmaMiriam Boersema
Nov 7, 2015·The Biochemical Journal·Lindsay R StolzenburgAnn Harris
Dec 15, 2015·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·L CalusP Gevaert
Dec 20, 2015·Biochemical and Biophysical Research Communications·Xin HeChunjiang Yu
Dec 20, 2015·International Journal of Immunopathology and Pharmacology·R LazzariniM Orciani
Dec 22, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Zhihong WangLiang He
Feb 3, 2016·JAMA : the Journal of the American Medical Association·Claus BachertE Rand Sutherland
Nov 5, 2016·Frontiers in Bioscience (Scholar Edition)·Maria D Castellone, Mikko O Laukkanen
Feb 25, 2017·American Journal of Rhinology & Allergy·Rafaela Veloso-Teles, Rui Cerejeira
Mar 10, 2017·Brazilian Journal of Otorhinolaryngology·Silke Anna Theresa WeberGiesela Fleischer Ferrari
Aug 15, 2017·International Journal of Pediatric Otorhinolaryngology·Melina FettaPetros V Vlastarakos

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Citations

Aug 28, 2021·International Journal of Molecular Sciences·Émile Bélanger, Catherine Laprise

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