Abstract
The fragile histidine triad (FHIT) tumor suppressor gene at 3p14.2 has abnormalities in several types of human cancers. To investigate the potential role of FHIT in cervical cancer, which exhibits frequent loss of heterozygosity of 3p, we have examined primary cervical cancer samples from 28 patients for alterations of the FHIT gene. Abnormal FHIT transcripts were detected using reverse transcription-polymerase chain reaction (PCR) and subsequently by sequencing. Of 28 primary cervical carcinomas analyzed, 12 tumors (43%) showed abnormal FHIT transcripts, including deletion, insertion and point mutation. Loss of a FHIT transcript was observed in 2 cases (7%). Allelic loss of the FHIT gene was detected in 16 of 27 informative cases (59%). Oncogenic human papillomavirus (HPV) type 16, 18, 33, 35, 58 and 59 were not only present but were expressed in 24 of 28 cases (85%) by consensus PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) analysis for the HPV E6 and E7 genes. Our data indicate that alteration of the FHIT gene is an important genetic event associated with cervical cancer and oncogenic
References
Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M ScheffnerP M Howley
May 1, 1991·The Journal of General Virology·Y FujinagaK Fujinaga
Apr 1, 1990·Human Genetics·N C PopescuJ A DiPaolo
May 16, 1990·Journal of the National Cancer Institute·S A FuquaW L McGuire
Oct 15, 1989·Cancer Genetics and Cytogenetics·N C Popescu, J A DiPaolo
Jan 1, 1988·The Journal of Experimental Medicine·D K ShibataW J Martin
Jul 1, 1988·Cancer Genetics and Cytogenetics·L A CannizzaroF Hecht
Feb 23, 1996·Cell·M OhtaK Huebner
Apr 5, 1996·Cell·G SozziC M Croce
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·L VirgilioC M Croce
Sep 10, 1996·Biochemistry·L D BarnesK Huebner
Feb 1, 1996·Human Molecular Genetics·C M WilkeT W Glover
Citations
Mar 27, 2003·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·C BaykalA Ayhan
Aug 25, 2009·Cancer Letters·Enrico GiarnieriRita Mancini
Dec 2, 2006·Human Pathology·Sun Och Yoon
May 23, 2008·Journal of Cutaneous Pathology·Sarah E GrayMohamed Mabruk
Sep 5, 2001·Gynecologic Oncology·F S LiuA J Lin
Sep 27, 2000·International Journal of Cancer. Journal International Du Cancer·A HellandR A Lothe
Dec 13, 2000·The Journal of Pathology·D ButlerE W Kay
May 17, 2000·Cancer Genetics and Cytogenetics·C MeninE D'Andrea
Oct 3, 2001·International Journal of Cancer. Journal International Du Cancer·Y HarimaT Ohnishi
Oct 21, 2005·World Journal of Gastroenterology : WJG·Po ZhaoYa-Li Lu
Jul 20, 2001·British Journal of Cancer·K OzakiY Murata
Mar 5, 2002·British Journal of Cancer·G TerryJ Cuzick
Sep 18, 2009·Cellular and Molecular Neurobiology·Gulsah CecenerKaya Aksoy
Dec 25, 2009·Journal of Cancer Research and Clinical Oncology·Md Imtaiyaz HassanFaizan Ahmad
Sep 2, 1999·British Journal of Cancer·P A Lazo
Apr 18, 2017·PloS One·Liming WangHui Wang
Jul 19, 2018·BioMed Research International·Weiyang LiDongmei Man
Dec 9, 2003·Ultrastructural Pathology·Qinghua WuJahn M Nesland
Sep 5, 2009·Molecular and Cellular Biochemistry·Md Kausar NeyazMausumi Bharadwaj