Abstract
Breast cancer is one of the leading causes of cancer-related death in women worldwide. Here we aimed to examine the expression status of S-phase kinase-associated protein 2 (Skp2) and p27(KIP1), and assess the significance of Skp2 plus p27(KIP1) expression in patients with intraductal proliferative lesions, including ductal carcinoma in situ (DCIS) and atypical ductal hyperplasia (ADH). Skp2 and p27(KIP1) mRNA levels in DCIS, ADH, flat epithelial atypia, and usual ductal hyperplasia (UDH) were evaluated by quantitative real-time reverse transcription polymerase chain reaction and protein expression was evaluated immunohistochemically in 60 fresh tissues and 120 paraffin-embedded tissues from patients with the four subtypes above. We found that the protein and mRNA level of Skp2 were significantly increased in DCIS and ADH as compared with that in UDH. In contrast, p27(KIP1) protein and mRNA levels were reduced. Based on the above findings, abnormal levels of Skp2 and p27(KIP1) have probably been involved in the pathogenesis of ADH and DCIS. Thus, Skp2 and p27(KIP1) may serve as important diagnosis markers.
References
May 14, 1999·Genes & Development·A MontagnoliM Pagano
Jun 27, 2001·The Journal of Cell Biology·A C Carrano, M Pagano
Jul 22, 2005·The New England Journal of Medicine·Lynn C HartmannDaniel W Visscher
Apr 26, 2006·Breast Cancer Research and Treatment·Frank TraubReinhard von Wasielewski
Jan 27, 2007·Cell·Isabel ChuJoyce Slingerland
Apr 14, 2007·Virchows Archiv : an International Journal of Pathology·Mohammad Reza KeikhaeeTakashi Takata
Apr 26, 2007·Molecular Pharmacology·Yi-He LingRoman Perez-Soler
May 8, 2007·Cancer Research·Maximilian ReichertGünter Schneider
Jun 15, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Amy C DegnimLynn C Hartmann
May 27, 2008·Nature Reviews. Cancer·David Frescas, Michele Pagano
Apr 25, 2009·The American Journal of Surgical Pathology·Quirino PiubelloAntonio Iannucci
Aug 13, 2011·AJNR. American Journal of Neuroradiology·A G OsbornM Castillo
Jan 27, 2012·Journal of Genetic Counseling·Shenela B Lakhani
Aug 1, 2010·Breast Care·Hans-Peter SinnSebastian Aulmann
Feb 26, 2013·AJR. American Journal of Roentgenology·Annalisa K BeckerChristine M Wilson
Sep 17, 2013·Endocrinology·Savvas C PavlidesLeslie I Gold
Feb 28, 2014·World Journal of Gastroenterology : WJG·Ja-Yeon KimJin Hee Sohn
Feb 3, 2015·Cancer·Samar M SaidAmy C Degnim
Mar 31, 2015·Nature Communications·Dazhi XuHui-Kuan Lin
Nov 4, 2016·Oncogene·S BhattB S Katzenellenbogen