Aberrant methylation inactivates somatostatin and somatostatin receptor type 1 in head and neck squamous cell carcinoma

PloS One
Kiyoshi MisawaHiroyuki Mineta

Abstract

The aim of this study was to define somatostatin (SST) and somatostatin receptor type 1 (SSTR1) methylation profiles for head and neck squamous cell carcinoma (HNSCC) tumors at diagnosis and follow up and to evaluate their prognostic significance and value as a biomarker. Gene expression was measured by quantitative RT-PCR. Promoter methylation status was determined by quantitative methylation-specific PCR (Q-MSP) in HNSCC. Methylation was associated with transcription inhibition. SST methylation in 81% of HNSCC tumor specimens significantly correlated with tumor size (P = 0.043), stage (P = 0.008), galanin receptor type 2 (GALR2) methylation (P = 0.041), and tachykinin-1 (TAC1) (P = 0.040). SSTR1 hypermethylation in 64% of cases was correlated with tumor size (P = 0.037), stage (P = 0.037), SST methylation (P < 0.001), and expression of galanin (P = 0.03), GALR2 (P = 0.014), TAC1 (P = 0.023), and tachykinin receptor type 1 (TACR1) (P = 0.003). SST and SSTR1 promoter hypermethylation showed highly discriminating receiver operator characteristic curve profiles, which clearly distinguished HNSCC from adjacent normal mucosal tissues. Concurrent hypermethylation of galanin and SSTR1 promoters correlated with reduced disease-free su...Continue Reading

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Citations

Jun 2, 2016·SpringerPlus·Chisako IzumiHiroyuki Mineta
Jul 19, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yuki MisawaHiroyuki Mineta
Jan 15, 2019·Expert Review of Molecular Diagnostics·Takeharu KanazawaThomas E Carey
Sep 21, 2018·Biomedical Reports·Daniela RussoGennaro Ilardi
Jan 27, 2017·Biomedical Reports·Karina Galoian, Parthik Patel
Oct 22, 2020·Reviews in Endocrine & Metabolic Disorders·M J KlompL J Hofland
Aug 11, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Rui-Lan HuangHung-Cheng Lai
Jan 26, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Wina VerlaatRenske D M Steenbergen

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Methods Mentioned

BETA
genotyping
PCR

Software Mentioned

StatMate

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