PMID: 16637262Apr 28, 2006Paper

Aberrant methylation of tumor suppressor genes and allelic imbalance in cervical intraepitelial neoplasia

Molekuliarnaia biologiia
T V KekeevaM V Nemtsova

Abstract

We analysed 42 high-grade CIN or CIN3 samples, 42 nondysplasia tissues adjacent to CIN3. 35 smears from women without gynecological pathology were also evaluated. Methylation status of six genes (p16, MLH1, HIC1, MGMT, N33 and RB1) was determined using methylation-sensitive PCR. There is some insignificant level of methylation determined in normal smears. Methylation percentages of the genes in CIN3 were: p16, 58%; MLH1, 51%; HIC1, 84%; N33, 27%. Methylation percentages of the genes in nondysplasia adjacent tissues were also high. There is no significant difference in methylation frequencies of MGMT and RB1 determined between dysplasia and control. We identified allelic imbalance at chromosomes 5q11-q14 and 13q14 in 21% cases (9/42). The incidence of LOH was investigated in 7% (3/42) cases at region 13q14.

References

Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·K HeselmeyerT Ried
Apr 20, 1999·Molecular and Cellular Probes·C SutterM von Knebel Doeberitz
May 3, 2001·Journal of the National Cancer Institute·D G BurbeeJ D Minna
Jul 16, 2002·Carcinogenesis·Esteban Ballestar, Manel Esteller
Dec 17, 2002·Molecular and Cellular Biology·Yutaka KondoJean-Pierre J Issa
Sep 26, 2003·The American Journal of Pathology·Sun LeeGyeong Hoon Kang
Feb 5, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Andreas WidschwendterMartin Widschwendter
Feb 12, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Rolf von KnoblochRainer Hofmann
Jul 15, 2004·Annals of the New York Academy of Sciences·Hannes M MüllerMartin Widschwendter
Nov 19, 2004·Nature·Neil A BhowmickHarold L Moses
Feb 17, 2005·Journal of the National Cancer Institute·Qinghua FengNancy B Kiviat

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.