Abstract
This study aimed to explore the underlying mechanism of protocadherin 17 (PCDH17) downregulated in nasopharyngeal cancer (NPC). NPC tumor and adjacent tissue samples were collected from NPC patients who received therapy in the Chinese PLA General Hospital. Meanwhile, a normal epithelial cell lines NP96 and 6 NPC and cell lines C666-1, CEN1, CEN2, HNE1, HEN2, and HONE1 were prepared. Then, the expression level of PCDH17 and the methylation level of PCDH17 promoter in both tissues samples and cell lines were determined using the PCR method. Moreover, PCDH17 was overexpressed in CNE2 and HONE1 using Lipo2000. Following this, the proliferation and apoptosis of CNE2 and HONE1 were assessed using MTT and flow cytometry. The expression of PCDH17 was significantly downregulated in NPC tissues compared with the adjacent tissues as well as in the NPC cell lines compared with the normal NP96 cells. Overexpressed PCDH17 could significantly inhibit the proliferation of CNE2 and HONE1 cells but obviously promote the apoptosis of these two cell lines. Aberrant hypermethylation in the promoter might be the explanation of PCDH17 downregulated in PCDH17 and promoted the development of NPC.
References
Dec 10, 2002·Mechanisms of Ageing and Development·Yasuhito Yuasa
Oct 26, 2005·Oncogene·J YingQ Tao
May 3, 2006·Cancer Research·Issei ImotoJohji Inazawa
Jan 21, 2009·International Journal of Radiation Oncology, Biology, Physics·Yan-Ping MaoJun Ma
Mar 5, 2010·Carcinogenesis·Shigeo HarukiJohji Inazawa
Jul 21, 2010·International Journal of Radiation Oncology, Biology, Physics·Shu-Zhen LaiJun Ma
Dec 22, 2010·Cell Adhesion & Migration·Soo-Young KimHyun Kim
Aug 19, 2011·Epigenetics : Official Journal of the DNA Methylation Society·Vera L CostaCarmen Jerónimo
Dec 31, 2011·Histology and Histopathology·Yueqin YangJi-Cheng Li
Jun 13, 2012·Seminars in Radiation Oncology·Anne W M LeeWai T Ng
Aug 29, 2012·The Journal of Pathology·Xiaotong HuChao He
Mar 22, 2013·Frontiers in Molecular Neuroscience·Joshua A Weiner, James D Jontes
Jul 11, 2013·British Journal of Cancer·E DaneseG Lippi
Dec 25, 2013·The Journal of International Medical Research·Zhen-Guo LuoJian-Guo Ma
Sep 1, 2015·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Meng-Xia ZhangMing-Yuan Chen
Sep 24, 2015·Molecular Nutrition & Food Research·Jia-Ching WuMin-Hsiung Pan
Sep 26, 2015·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Ying-Li LinWen-Ping Li
Dec 20, 2015·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Ying-Li LinWen-Ping Li
May 20, 2016·Cold Spring Harbor Perspectives in Biology·Stephen B Baylin, Peter A Jones
May 20, 2016·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Zhiwei LiuWeimin Ye
Jun 29, 2016·Oncotarget·Xuedong YinQian Tao
Sep 20, 2016·Pediatric Blood & Cancer·Thanh Nha UyenKenichi Koike
Oct 25, 2016·Cancer Medicine·Chun ChenRaoying Xie
Jul 9, 2017·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Ying-Li LinXu Zhang