Aberrant retinal tight junction and adherens junction protein expression in an animal model of autosomal dominant Retinitis pigmentosa: the Rho(-/-) mouse

Experimental Eye Research
M CampbellBrenda Brankin

Abstract

Retinitis pigmentosa (RP) comprises a heterogeneous group of inherited diseases that are characterised by primary degeneration of rod photoreceptors and secondary degeneration of cone photoreceptors in the retina. Additional pathological changes include vascular changes and invasion of the inner retina by retinal pigment epithelial (RPE) cells. RP represents a major cause of progressive retinal disease worldwide. Using a mouse model of autosomal dominant Retinitis pigmentosa (adRP) with retinopathy induced by targeted disruption of the rhodopsin gene Rho(-/-), we have analysed the levels of expression of a range of tight and adherens junction associated proteins, in order to further elucidate the pathogenic mechanisms occurring at an early stage of this condition. Using western blot analysis and indirect immunostaining of retinal cryosections from 6-week-old mice from a C-129 background we have determined changes, if any, in the levels of expression and localisation of a series of tight and adherens junction associated proteins, including Zonula Occludens-1 (ZO-1), occludin, N-Cadherin, p120-Catenin, alpha-Catenin, gamma-Catenin, beta-Catenin, and E-Cadherin. We have found an up-regulation of the tight junction and adherens jun...Continue Reading

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Citations

Aug 29, 2007·Investigative Ophthalmology & Visual Science·Kenneth J MandellCharles A Parkos
Dec 19, 2012·Proceedings of the National Academy of Sciences of the United States of America·Amanda C BarberRachael A Pearson
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Jan 2, 2020·International Journal of Molecular Sciences·Aisling NaylorMatthew Campbell
Dec 13, 2017·Progress in Retinal and Eye Research·Richard F SpaideGiovanni Staurenghi
Dec 20, 2021·The FEBS Journal·Fionn O'Leary, Matthew Campbell

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