Aberrant transposition of a Tc1-mariner element, impala, in the fungus Fusarium oxysporum

Molecular Genetics and Genomics : MGG
A Hua-VanM-J Daboussi

Abstract

We previously determined that the impalaD transposable element of Fusarium oxysporum was able to mobilize a non autonomous copy of impala ( niaD::imp::hph), inserted in the niaD gene encoding nitrate reductase. Generally, mobilization results in the recovery of Nia(+) revertants at low frequency. In the course of this study, we recovered a transformant that gave rise to Nia(+) revertants at a high rate. These revertants displayed atypical phenotypes and showed a niaD hybridization pattern different from that in more typical revertants. Molecular analysis of the structure of the transformant and atypical revertants indicated that (i) in the transformant, two copies of impala, one defective and one active, were inserted at the same genomic locus in a head-to-head orientation; and (ii) all the revertants analyzed presented the same chromosomal rearrangement, an inversion resulting in the replacement of the niaD promoter by a new sequence containing a cryptic promoter. We also frequently observed additional DNA rearrangements (deletion or inversion) in these revertants. The sequences at the rearrangement junctions indicated the occurrence of a transposition event that used the ITRs (Inverted Terminal Repeats) of separate transposon...Continue Reading

Citations

May 8, 2003·Molecular Genetics and Genomics : MGG·O LadendorfJ Kämper
Oct 2, 2008·Nature Reviews. Drug Discovery·Rahul Kakkar, Richard T Lee
Feb 10, 2006·Cell Research·Richard J WeldHayley J Ridgway
Oct 4, 2011·Nucleic Acids Research·Yuan Hu XuanChang-deok Han
Mar 21, 2009·Genes & Development·Jianbo ZhangThomas Peterson
Oct 13, 2006·PLoS Genetics·Jianbo ZhangThomas Peterson
Sep 6, 2007·PloS One·Mamadou B CoulibalyNora J Besansky
Dec 14, 2007·Annual Review of Genetics·Cédric Feschotte, Ellen J Pritham
Jun 14, 2014·Mobile DNA·Yuan Liu, Guojun Yang
May 13, 2005·Current Genetics·Caroline B MichielseArthur F J Ram
Sep 22, 2010·Fungal Genetics and Biology : FG & B·A John Clutterbuck
Oct 7, 2003·Annual Review of Microbiology·Marie-Josée Daboussi, Pierre Capy
Mar 29, 2002·Molecular Biology and Evolution·Marie-Josée DaboussiThierry Langin
Apr 28, 2021·Genetics·Sharu Paul SharmaThomas Peterson
May 7, 2009·Genes & Development·Clifford F Weil

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.