Ability of an ELISA-Based Seed Health Test to Detect Erwinia stewartii in Maize Seed Treated with Fungicides and Insecticides

Plant Disease
J K PatakyD G White

Abstract

Two sets of experiments were done to examine whether seed-treatment chemicals affected the ability of an enzyme-linked immunosorbent assay (ELISA)-based seed health test to detect Erwinia stewartii. The chemicals evaluated included Actellic, Apron, Captan, Cruiser, Gaucho, Maxim, Poncho, Thiram, and Vitavax in 11 seed-treatment combinations. In one experiment, seed-treatment chemicals were evaluated quantitatively in a critical region of ELISA absorbance values near 0.5 using maize seed that were spiked with uniform quantities of a liquid suspension of E. stewartii. The number of bacteria in each sample was estimated from ELISA absorbance values using standard curves. Log CFU of E. stewartii per sample were not significantly different among the untreated control and the 11 seed treatments compared with Tukey's Studentized Range Test (P = 0.05). Means of log CFU/ml for all treatments were tightly clustered around 5.70 which corresponded to an absorbance value of 0.440 and a bacterial population of about 500,000 CFU/ml. In a second set of experiments, seed treatment chemicals were evaluated based on qualitative decisions that resulted from the ELISA-based seed health test of seed lots of Jubilee and A632 infected with E. stewarti...Continue Reading

Citations

Aug 24, 2020·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Junli DuanGuina Xiao

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