Ability of Escherichia coli to produce hemolysis leads to a greater pathogenic effect on human sperm

Fertility and Sterility
Rodrigo BoguenJ Villegas

Abstract

To determine the effect on human sperm of Escherichia coli strains separated on the basis of their ability to produce hemolysis. Experimental study. University-based laboratory. Semen samples from healthy donors. Five million sperm, selected via the swim-up method, were incubated with 3 E. coli concentrations to obtain ratios of sperm to E. coli of 1:2, 1:16, and 1:128. The E. coli strains were: a hemolytic isolated strain (H), a nonhemolytic American Type Culture Collection strain (NH-ATCC), and a nonhemolytic isolated strain (NH-I). Aliquots of human sperm were used to measure progressive motility using computer-aided sperm analysis, mitochondrial membrane potential (ΔΨm) with a JC-1 (5,5',6,6' tetrachloro-1,1',3,3'-tetraethylbenzamidazolocarbocyanin iodide) and propidium iodide stain, and intracellular reactive oxygen species (iROS) with a dihydroethidium (DHE) stain. Sperm ΔΨm and iROS were measured by flow cytometry. Sperm vitality was considered the mean of propidium iodide-negative and DHE-negative cells. Sperm incubated with the H strain in a 1:2 sperm to bacteria ratio demonstrated a significant decrease in motility and ΔΨm, and an increase of iROS. The NH-ATCC strain decreased sperm motility and ΔΨm, but in a ratio of...Continue Reading

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Citations

Dec 21, 2018·Andrologia·Ruben D MotrichVirginia E Rivero
Dec 21, 2018·Andrologia·Ashok AgarwalRalf Henkel
Jun 13, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Pamela UribeRaúl Sánchez
Feb 12, 2021·Frontiers in Cellular and Infection Microbiology·Fenghao ZhangTingtao Chen
May 25, 2021·Clinical and Experimental Reproductive Medicine·Khadije Rezai KhanmohammadFateme Zare
Sep 2, 2021·Frontiers in Cellular and Infection Microbiology·Chunlei MeiDonghui Huang
May 23, 2018·Gynécologie, Obstétrique, Fertilité & Sénologie·H HannachiO Bahri
Dec 2, 2021·Antioxidants & Redox Signaling·Sara Escada-RebeloSandra Amaral

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